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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

Plasmid

#113886

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

Plasmid

#113890

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

Plasmid

#83286

Purpose

untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

mTurquoise2-FHA2-AURKB substrate-YPet

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

Plasmid

#163610

Purpose

Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2-optoNES-WPRE

Plasmid

#159962

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2-optoNES

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mTurquoise-P2A-iCre (JDW 920)

Plasmid

#224518

Purpose

A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

mTurquoise-P2A-iCre

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE

Plasmid

#159941

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2-optoNES

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mTurquoise2-optoJNKi-V416I-WPRE

Plasmid

#159970

Purpose

optogenetic JNK inhibitor with extended relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mTurquoise2-optoJNKi-V416I

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mTurquoise2-optoJNKi-C450A-WPRE

Plasmid

#159969

Purpose

negative control (non-activatable) optogenetic JNK inhibitor

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mTurquoise2-optoJNKi-C450A

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

C450A

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mTurquoise2-3XNLS-optoNES-WPRE

Plasmid

#159946

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mTurquoise2-3XNLS-optoNES

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

Plasmid

#198753

Purpose

Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

Depositor

Jacco van Rheenen

Article

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

See depositor comments below

Promoter

EF1a

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

Plasmid

#129650

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Use

Tags

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-P2A-Pre-SPHERE-sfmTurquoise2-iGluSnFR

Plasmid

#187095

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-sfmTurquoise2-iGluSnFR

Use

Tags

mCherry-P2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Plasmid

#226514

Purpose

Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

Depositor

Kai Johnsson

Article

Kühn et al bioRxiv 2024.08.28.610127

Insert

HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

Plasmid

#85146

Purpose

lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mRuby3-P2A-mTurquoise-MLC

Use

Lentiviral

Tags

mRuby3, mTurquoise

Expression

Mutation

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159961

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159965

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159964

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159958

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159957

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge