-
Plasmid#141313PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pCDH-2xMARS-nGFP-puro
Plasmid#205238PurposeLentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobodyDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseLentiviralTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRATIO3212
Plasmid#141311PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRATIO4214
Plasmid#141314PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmCerulean and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-On-HTR6-RhoA sensor
Plasmid#189614PurposeTet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensorDepositorInsertHTR6 (HTR6 Human)
UseTagsGFP and mScarletExpressionMammalianMutationPromoterTet3GAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP5
Plasmid#209960PurposeEntry vector to store Level 0* resistance cassette partsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP2
Plasmid#209989PurposeEntry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRE145
Plasmid#118497Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE155
Plasmid#118505Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE135
Plasmid#118489Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE165
Plasmid#118513Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP8823
Plasmid#220901PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8835
Plasmid#220902PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8921
Plasmid#220903PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8923
Plasmid#220904PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPR3
Plasmid#209986PurposeContains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmidsDepositorInsertresistance cassette (ermBL)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCS111
Plasmid#209332PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
IBMc120
Plasmid#161950PurposeContains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmidDepositorInsertpSB4A3-PECF20-B30-mScarlet-I-B15
UseTagsExpressionBacterialMutationPromoterPECF20Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc393
Plasmid#161953PurposeDemonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmidDepositorInsertpSB4A3-T-PT7-B30-mScarlet-I-3T
UseTagsExpressionBacterialMutationPromoterPT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only