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Plasmid#174715PurposeStable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutationsDepositorInsertMeikin
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation1-332, E151R, R154EPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]
Plasmid#196887PurposeDual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotrasfection reporterDepositorInsertroxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template
Plasmid#214999PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PDK-198}:NosT
Plasmid#212170PurposeThis binary vector expresses mNeonGreen containing the castor bean catalase (PDK) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PDK-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PDK int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-198}:NosT
Plasmid#212172PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-216}:NosT
Plasmid#212173PurposeThis binary vector expresses mNeonGreen containing the PIV intron (216 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-216}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-390}:NosT
Plasmid#212174PurposeThis binary vector expresses mNeonGreen containing the PIV intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-390}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{AGO-198}:NosT
Plasmid#212175PurposeThis binary vector expresses mNeonGreen containing the Argonaut (AGO) intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{AGO-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and AGO int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{PDK-198}:NosT
Plasmid#212176PurposeThis binary vector expresses mNeonGreen containing the catalase (PDK) intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PDK-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PDK int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PIV-198}:NosT
Plasmid#212166PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PIV-216}:NosT
Plasmid#212167PurposeThis binary vector expresses mNeonGreen containing the PIV intron (216bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-216}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PIV-390}:NosT
Plasmid#212168PurposeThis binary vector expresses mNeonGreen containing the PIV intron (390bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-390}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{AGO-198}:NosT
Plasmid#212169PurposeThis binary vector expresses mNeonGreen containing the Argonaut (AGO) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{AGO-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and AGO int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen
Plasmid#211692PurposeU6-sgRNA-EFS-Cas9-P2A-mNeonGreenDepositorInsertSpyCas9 and guide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)
Plasmid#193854PurposePromoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing padDepositorInsert5' Cbr-unc-119(+) + 5' mNeonGreen
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterCbr-unc-119pAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)
Plasmid#173462PurposeC-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)
Plasmid#173460PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1
Plasmid#196890PurposeNeuron-specific expression of human (h)Centrin fused to mNeonGreenDepositorInsertmNeonGreen-hCentrin1 (CETN1 Human)
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE mNeonGreen-Core (HBc from HBV) IRES puro
Plasmid#122202PurposePlasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.DepositorInsertsHBc from HBV AYW
mNeonGreen
UseTagsGGSGGSGGS linkerExpressionMammalianMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
Plasmid#132402Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only