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Plasmid#224379PurposeTargets TELO2 for dTAG N terminal knock-in cell linesDepositorInsertTELO2 (TELO2 Human)
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459_GNB1L_gRNA
Plasmid#224377PurposeTargets GNB1L for dTAG C terminal knock-in cell linesDepositorInsertGNB1L (GNB1L Human)
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR_AtU6p_BsaI_ccdb_gRNA
Plasmid#225986PurposeTo make Gateway entry clone of CRISPR guide RNA under AtU6pDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
gRNA_SRR124.5_tagBFP
Plasmid#163753PurposegRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.DepositorInsertgRNA_SRR124.5
UseCRISPRTagstagBFPExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA_SRR134.3_miRFP670
Plasmid#163754PurposegRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.DepositorInsertgRNA_SRR134.3
UseCRISPRTagsmiRFP670ExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA_CloningVector_tagBFP
Plasmid#163747PurposeEmpty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA_CloningVector_miRFP670
Plasmid#163748PurposeEmpty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA_SOX2_HDR
Plasmid#163752PurposegRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.DepositorInsertgRNA_SOX2 (SOX2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p105_gRNA_Sa_CD40
Plasmid#213781PurposeExpresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.DepositorInsertSa gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA TR KO 2
Plasmid#207610PurposesgRNA 2 to knockout TR by replacement with a PuroR cassetteDepositorInsertTCAGGCCGCAGGAAGAGGAA
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA TR KO 1
Plasmid#207609PurposesgRNA 1 to knockout TR by replacement with a PuroR cassetteDepositorInsertACCCTAACTGAGAAGGGCGT
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
UCK2_Deletion_gRNA_1
Plasmid#204672PurposeDual gRNA plasmid for UCK2 deletionDepositorInserturidine-cytidine kinase 2 (UCK2 Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
UCK2_Deletion_gRNA_2
Plasmid#204673PurposeDual gRNA plasmid for UCK2 deletionDepositorInserturidine-cytidine kinase 2 (UCK2 Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_T1snp_gRNA
Plasmid#213789PurposepDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNADepositorInsertnrg1 type I SNP sgRNA (nrg1 Zebrafish)
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_T1_gRNA
Plasmid#199387PurposepDEL111; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I sgRNADepositorInsertnrg1 type I sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_T2_gRNA
Plasmid#199388PurposepDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNADepositorInsertnrg1 type II sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_Ex3_1
Plasmid#210625PurposegRNA target sequences for TAL1 Exon 3DepositorInsertTAL1 Exon 3 gRNA (TAL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_Ex3_2
Plasmid#210626PurposegRNA target sequences for TAL1 Exon 3DepositorInsertTAL1 Exon 3 gRNA (TAL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_Ex3_3
Plasmid#210627PurposegRNA target sequences for TAL1 Exon 3DepositorInsertTAL1 Exon 3 gRNA (TAL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_Ex3_4
Plasmid#210628PurposegRNA target sequences for TAL1 Exon 3DepositorInsertTAL1 Exon 3 gRNA (TAL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only