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pGTag-TagRFP-CAAX-B-actin

Plasmid

#117810

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Actin binding domain of Ezrin

Plasmid

#62381

Purpose

Expresses the actin binding domain of ezrin. Amino acids 552-586.

Depositor

Guillaume Charras

Article

Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

Insert

Actin binding domain of ezrin (EZR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains aa's 552-586

Promoter

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-TagRFP-B-actin

Plasmid

#117806

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-mScarlet-Dync1h1motor

Plasmid

#191330

Purpose

Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-eGFP-B-actin

Plasmid

#117812

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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Actin-53a-ires-EGFP

Plasmid

#25460

Depositor

Jerry Crabtree

Article

Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

Insert

BAF53a (Actl6a Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pBetaActin-KIF13aMotor-mScarlet-FKBP

Plasmid

#191337

Purpose

Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

KIF13aMotor-mScarlet-FKBP (KIF13A Human)

Use

Tags

Expression

Mammalian

Mutation

motor domain aa 1-639

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-CAAX-B-actin

Plasmid

#117816

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Contactin/F3 [K73/20R]

Plasmid

#177447

Purpose

Mammalian Expression Plasmid of anti-Contactin/F3 (Rat). Derived from hybridoma K73/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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eTC GFP ß-actin ΔZip

Plasmid

#27124

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Use

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

This construct lacks β-actin 3′UTR and therefore …

Promoter

Available since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only

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Mutant Human Cortactin-GFP

Plasmid

#71365

Purpose

mammalian expression of mutant human Cortactin-GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

cortactin (CTTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

silent mutation present at 72nd nucleotide

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only
Actin-53b-ires-EGFP

Plasmid

#25459

Depositor

Jerry Crabtree

Article

Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

Insert

BAF53b (Actl6b Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-EGFP

Plasmid

#164940

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Expression

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pME mKate2-dynactin1-811

Plasmid

#105971

Purpose

entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-Actin/pEGFP-C1

Plasmid

#51972

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

Actin

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
hsp70: mKate2 dynactin1-811

Plasmid

#105970

Purpose

heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Actin-BAF53(b+a)-ires-EGFP

Plasmid

#25462

Depositor

Jerry Crabtree

Article

Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

Insert

BAF53(b+a)

Use

Tags

EGFP

Expression

Mammalian

Mutation

3' of mBAF53b deleted and fused to 3' o…

Promoter

Available since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only

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Actin-BAF53(a+b)-ires-EGFP

Plasmid

#25461

Depositor

Jerry Crabtree

Article

Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

Insert

BAF53(a+b)

Use

Tags

EGFP

Expression

Mammalian

Mutation

3' of mBAF53a deleted and fused to 3' o…

Promoter

Available since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only

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ACTIN-Cxcl5-PGK-Cre

Plasmid

#110278

Purpose

Bicistronic lentivirus expressing Cxcl5 and Cre

Depositor

Trudy Oliver

Article

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Inserts

chemokine (C-X-C motif) ligand 5 (Cxcl5 Mouse)
cyclization recombinase

Use

Cre/Lox and Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

B-actin and Pgk

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only
pENTR-actin-G168D,Y169D

Plasmid

#118384

Purpose

Gateway compatible donor vector with actin-G168D,Y169D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

G168D,Y169D

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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