-
Plasmid#62381PurposeExpresses the actin binding domain of ezrin. Amino acids 552-586.DepositorInsertActin binding domain of ezrin (EZR Human)
UseTagsGFPExpressionMammalianMutationcontains aa's 552-586PromoterAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
RHPMP132 (mEOS3.1-beta Actin)
Plasmid#88836PurposeLocalization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1DepositorInsertbeta-Actin (ACTB Human)
UseTagsmEOS3.1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-KIF13aMotor-mScarlet-FKBP
Plasmid#191337PurposeExpresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertKIF13aMotor-mScarlet-FKBP (KIF13A Human)
UseTagsExpressionMammalianMutationmotor domain aa 1-639PromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-B-actin
Plasmid#117812PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Actin-53a-ires-EGFP
Plasmid#25460DepositorInsertBAF53a (Actl6a Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-CAAX-B-actin
Plasmid#117816PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsCAAXExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Contactin/F3 [K73/20R]
Plasmid#177447PurposeMammalian Expression Plasmid of anti-Contactin/F3 (Rat). Derived from hybridoma K73/20.DepositorInsertanti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntn1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
eTC GFP ß-actin ΔZip
Plasmid#27124DepositorInsertbeta-actin (ACTB Human)
UseTagsEGFP and TC tagExpressionMammalianMutationThis construct lacks β-actin 3′UTR and therefore …PromoterAvailable sinceJan. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
Mutant Human Cortactin-GFP
Plasmid#71365Purposemammalian expression of mutant human Cortactin-GFPDepositorInsertcortactin (CTTN Human)
UseTagsEGFPExpressionMammalianMutationsilent mutation present at 72nd nucleotidePromoterCMVAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Actin-53b-ires-EGFP
Plasmid#25459DepositorInsertBAF53b (Actl6b Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-EGFP
Plasmid#164940PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminusDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPExpressionMutationStop codon deletedPromoteractinb (actb2) promoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME mKate2-dynactin1-811
Plasmid#105971Purposeentry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811DepositorInsertdynactin1-811 (DCTN1 Human)
UseTagsmKate2ExpressionBacterialMutationcontains only amino acids 1-811PromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-Actin/pEGFP-C1
Plasmid#51972Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actinDepositorInsertNano-lantern
UseTagsActinExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
hsp70: mKate2 dynactin1-811
Plasmid#105970Purposeheat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein functionDepositorInsertdynactin1-811 (DCTN1 Human)
UseTagsmKate2ExpressionBacterialMutationcontains only amino acids 1-811PromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Actin-BAF53(b+a)-ires-EGFP
Plasmid#25462DepositorInsertBAF53(b+a)
UseTagsEGFPExpressionMammalianMutation3' of mBAF53b deleted and fused to 3' o…PromoterAvailable sinceSept. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
Actin-BAF53(a+b)-ires-EGFP
Plasmid#25461DepositorInsertBAF53(a+b)
UseTagsEGFPExpressionMammalianMutation3' of mBAF53a deleted and fused to 3' o…PromoterAvailable sinceSept. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
ACTIN-Cxcl5-PGK-Cre
Plasmid#110278PurposeBicistronic lentivirus expressing Cxcl5 and CreDepositorInsertsUseCre/Lox and LentiviralTagsNo fusion genesExpressionMammalianMutationWild typePromoterB-actin and PgkAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-G168D,Y169D
Plasmid#118384PurposeGateway compatible donor vector with actin-G168D,Y169D.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationG168D,Y169DPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBactin-PACT-mOrange2-hCM1
Plasmid#196868PurposeExpression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosomeDepositorInsertPACT-mOrange2-hCM1 (Akap9 Rat)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mOrange2-Cdk5rap2-CTD
Plasmid#196855PurposeExpression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInsertmOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only