eTC GFP ß-actin ΔZip
(Plasmid #27124)

• Depositing Lab: Robert Singer
• Publication: Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-actin
• Species: H. sapiens (human)
• Mutation: This construct lacks β-actin 3′UTR and therefore the zipcode sequence
• Entrez Gene: ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
• Tags / Fusion Proteins:
  • TC tag (N terminal on insert)
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene's sequencing results indicate the presence of several nucleotide inserts (starting at bp# 2459, 2501 and 2516) when compared to the sequence provided by the depositing laboratory. The depositing scientist confirmed that our sequencing result is correct and the inserts do not affect the function of the plasmid.