Skip to main content
Addgene
Showing: 301 - 320 of 14026 results

  1. pMT494

    Plasmid
    #139399
    Purpose
    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139399-plasmid-map-sequence-id-313941

    Enlarge

  2. pMT449

    Plasmid
    #139389
    Purpose
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139389-plasmid-map-sequence-id-313839

    Enlarge

  3. pMT455

    Plasmid
    #139392
    Purpose
    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    aTC and BA inducible promoters
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139392-plasmid-map-sequence-id-313930

    Enlarge

  8. pET28a Gx-NL3

    Plasmid
    #169108
    Purpose
    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169108-plasmid-map-sequence-id-335659

    Enlarge

  9. pMT469

    Plasmid
    #139395
    Purpose
    Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    aTC and BA inducible promoters
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    139395-plasmid-map-sequence-id-313937

    Enlarge

  11. FB398

    Plasmid
    #203634
    Purpose
    Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).
    Depositor
    Jose Marcos
    Article
    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1
    Insert
    FB367+FB395
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    203634-plasmid-map-sequence-id-410738

    Enlarge

  14. pMT468

    Plasmid
    #139394
    Purpose
    Nanoluc placed on Output 2 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Nanoluc placed on Output 2 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    aTC and BA inducible promoters
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139394-plasmid-map-sequence-id-313946

    Enlarge

  17. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Use
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

    Enlarge

  20. FB399

    Plasmid
    #203635
    Purpose
    Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).
    Depositor
    Jose Marcos
    Article
    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1
    Insert
    FB367+FB396
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    203635-plasmid-map-sequence-id-410739

    Enlarge

Showing: 301 - 320 of 14026 results