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MK1274

Plasmid

#71431

Purpose

This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

Depositor

Howard Salis

Article

Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

Insert

Mixed Feedback Loop version of the UBER system

Use

Tags

Expression

Mutation

T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

Promoter

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-L1221

Plasmid

#101335

Purpose

negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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OA-1050G (white)

Plasmid

#132420

Purpose

express arrays of gRNA targeting White under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

white gRNA array

Use

Tags

Expression

Insect

Mutation

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF2-CDS

Plasmid

#136040

Purpose

UPF2 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCGTTATGTTTGGTGGAAGAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF2 (UPF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM sh2 DNMT1

Plasmid

#88885

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.2 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTGMP-p16/p19.478

Plasmid

#32717

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

p16/p19

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AagRNA

Plasmid

#121953

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b tracrRNA/crRNA duplex

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF2-3'UTR

Plasmid

#136041

Purpose

UPF2 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CGCGAGGGTTAATCTTCTCTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF2 (UPF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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BC1364-mouse U6-sgH2B

Plasmid

#164043

Purpose

U6-driven sgRNA targeting H2B

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

H2B targeting sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AmsgRNA

Plasmid

#121956

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AmCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_SaCas9_sgRNA2

Plasmid

#107330

Purpose

U6 driven SaCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF331_1

Plasmid

#135739

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Drosha_3

Plasmid

#79862

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pLKO-cfNHERF2_5

Plasmid

#195228

Purpose

shRNA to canine NHERF2.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

NHERF2 (SLC9A3R1 canine)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-GeneTRAP

Plasmid

#218187

Purpose

To KO and genetrap mouse Vamp2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-Tag-HA

Plasmid

#218188

Purpose

To tag endogenous mouse VAMP2 with HA

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgFADD

Plasmid

#211527

Purpose

Deletes FADD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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