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Showing: 751 - 800 of 1002 results

  1. pQlox66R

    Plasmid
    #135657
    Purpose
    Clostridial vector, encoding a targetron carrying a lox66 site in the reverse (antisense) orientation. Retarget intron to insert lox66 onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135657-plasmid-map-sequence-id-305684

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  2. pQadd1R

    Plasmid
    #135661
    Purpose
    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135661-plasmid-map-sequence-id-305685

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  3. pQlox71R

    Plasmid
    #135656
    Purpose
    Clostridial vector, encoding a targetron carrying a lox71 site in the reverse (antisense) orientation. Retarget intron to insert lox71 onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135656-plasmid-map-sequence-id-305681

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  4. pQlox71F

    Plasmid
    #135655
    Purpose
    Clostridial vector, encoding a targetron carrying a lox71 site in the forward (sense) orientation. Retarget intron to insert lox71 onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135655-plasmid-map-sequence-id-305679

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  5. pQlox66F

    Plasmid
    #135658
    Purpose
    Clostridial vector, encoding a targetron carrying a lox66 site in the forward (sense) orientation. Retarget intron to insert lox66 onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    135658-plasmid-map-sequence-id-275772

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  13. pQadd1F

    Plasmid
    #135660
    Purpose
    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135660-plasmid-map-sequence-id-305687

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  15. pAAV-hSyn-Flex-Axon-EGFP

    Plasmid
    #135429
    Purpose
    Can be used to drive axon-enriched expression of EGFP in the presence of Cre recombinase.
    Depositor
    Rylan Larsen
    Has Service
    AAV1
    Insert
    axon-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GAP43 palmitoylation domain
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135429-plasmid-map-sequence-id-271167

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  17. mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177220
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177220-plasmid-map-sequence-id-350037

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  18. mU6-sgNT-hU6-sgLkb1_1st

    Plasmid
    #177221
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNT1/sgLkb1_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    177221-plasmid-map-sequence-id-363973

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  19. mU6-sgNT-hU6-sgLkb1_2nd

    Plasmid
    #177223
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNT1/sgLkb1_2nd
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177223-plasmid-map-sequence-id-351959

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  20. mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177224
    Purpose
    Expresses Ampk1 and Ampk2 targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177224-plasmid-map-sequence-id-351957

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  21. mU6-sgLkb1_2nd-hU6-sgNT

    Plasmid
    #177222
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    177222-plasmid-map-sequence-id-350113

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  22. mU6-sgSik1_2nd-hU6-sgSik3_2nd

    Plasmid
    #177219
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_2nd/sgSik3_2nd
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177219-plasmid-map-sequence-id-350035

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  23. mU6-sgSik1_2nd-hU6-sgSik3_1st

    Plasmid
    #177218
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_2nd/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177218-plasmid-map-sequence-id-350031

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  24. mU6-sgSik1_1st-hU6-sgSik3_2nd

    Plasmid
    #177217
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik3_2nd
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177217-plasmid-map-sequence-id-350029

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  25. mU6-sgSik1_1st-hU6-sgSik3_1st

    Plasmid
    #177216
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177216-plasmid-map-sequence-id-350028

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  26. mU6-sgSik3_2nd-hU6-sgNT

    Plasmid
    #177215
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik3_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177215-plasmid-map-sequence-id-350026

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  27. mU6-sgSik3_1st-hU6-sgNT

    Plasmid
    #177214
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik3_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177214-plasmid-map-sequence-id-349897

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  28. mU6-sgSik1_2nd-hU6-sgNT

    Plasmid
    #177213
    Purpose
    Expresses a Sik1-targeting (mU6) and a non-targeting (hU6) gRNAs and Cre recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177213-plasmid-map-sequence-id-349895

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  29. mU6-sgSik1_1st-hU6-sgNT

    Plasmid
    #177212
    Purpose
    Expresses a Sik1-targeting (mU6) and non-targeting (hU6) gRNAs, and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177212-plasmid-map-sequence-id-349892

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  30. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

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  31. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113700
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113700-plasmid-map-sequence-id-231558

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  32. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113687
    Purpose
    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113687-plasmid-map-sequence-id-231825

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  33. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113688
    Purpose
    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113688-plasmid-map-sequence-id-231828

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  34. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  35. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113689
    Purpose
    SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113689-plasmid-map-sequence-id-231823

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  36. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113701
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113701-plasmid-map-sequence-id-232036

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  37. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113699
    Purpose
    Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113699-plasmid-map-sequence-id-229551

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  38. pCALNL-GFP

    Plasmid
    #13770
    Purpose
    Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    EGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13770-plasmid-map-sequence-id-7759

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  40. pRVV700

    Plasmid
    #119017
    Purpose
    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9
    Depositor
    Richard Mann
    Article
    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.
    Insert
    TP901-1 attP sites
    Use
    CRISPR; Donor vector for creation of platforms fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    119017-plasmid-map-sequence-id-232904

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  41. pRVV609

    Plasmid
    #119014
    Purpose
    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9
    Depositor
    Richard Mann
    Article
    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.
    Insert
    TP901-1 attP sites
    Use
    CRISPR; Donor vector for creation of platforms fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119014-plasmid-map-sequence-id-232795

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  42. pRVV688

    Plasmid
    #119015
    Purpose
    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9
    Depositor
    Richard Mann
    Article
    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.
    Insert
    TP901-1 attP sites
    Use
    CRISPR; Donor vector for creation of platforms fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119015-plasmid-map-sequence-id-232899

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  43. pRVV689

    Plasmid
    #119016
    Purpose
    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9
    Depositor
    Richard Mann
    Article
    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.
    Insert
    TP901-1 attP sites
    Use
    CRISPR; Donor vector for creation of platforms fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119016-plasmid-map-sequence-id-232901

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  49. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

    Plasmid
    #115518
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    eGFP
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    115518-plasmid-map-sequence-id-229325

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  50. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112668
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112668-plasmid-map-sequence-id-229324

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Showing: 751 - 800 of 1002 results