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Plasmid#177077PurposeMoClo Level 1, position 5, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsertiridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0792
Plasmid#177076PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0786
Plasmid#177071PurposeMoClo Level 1, position 3, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0785
Plasmid#177070PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsert1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0768
Plasmid#177058PurposeMoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0124
Plasmid#177056PurposeMoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0120
Plasmid#177052PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0861
Plasmid#177078PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsert7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0796
Plasmid#177074PurposeMoClo Level 1, position 4, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsert8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0065
Plasmid#177019PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsert1-deoxy-D-xylulose 5-phosphate synthase
UseSynthetic BiologyTagsExpressionMutationtransit peptide removed, amplified from cDNA, con…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265C
Plasmid#164715PurposeGateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editingDepositorInserthAID-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible haid-cbe_v01 entry clo…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E1
Plasmid#164718PurposeGateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editingDepositorInsertA3A-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible a3a-cbe_v01 entry cloneTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E3
Plasmid#164720PurposeGateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editingDepositorInserteA3A-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible ea3a-cbe_v01 entry clo…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_dead_mt
Plasmid#167270PurposeOverexpression of the dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAP-tag
UseTagsHis x10ExpressionBacterialMutationC145APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast_dead_mt
Plasmid#167272PurposeOverexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
UseTagsHis x10ExpressionBacterialMutationE30R-C145APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA3
Plasmid#158481PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E, STOP codonPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA2
Plasmid#158480PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationD489V, STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA11
Plasmid#158489PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E no STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA9
Plasmid#158487PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationno STOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only