We narrowed to 9,511 results for: try

1. KCC2B_HUMAN_D0

   Plasmid

   #79718

   Purpose

   This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KCC2B
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. LentiGuide-Puro-CTRLg1 (CG509)

   Plasmid

   #139455

   Purpose

   Lentiviral vector with non-targeting gRNA and puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   Non-targeting sgRNA inserted; resistance gene: puroR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MK14_HUMAN_D0

   Plasmid

   #79680

   Purpose

   This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK14
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-DONSON

   Plasmid

   #208797

   Purpose

   Xenopus laevis His6-Myc-DONSON wild type

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Tags

   His and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ166-iSpyMac

   Plasmid

   #149553

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   iSpyMacCas9
   

   Use

   CRISPR; Gateway compatible zispymaccas9 entry clo…

   Tags

   3X FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   R221K, N394K, Protospacer interacting domain form…

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

   Plasmid

   #167268

   Purpose

   Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HOB
   

   Tags

   His x10

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. IG510: pMVP (R4-R3) human PDX1

   Plasmid

   #121744

   Purpose

   pMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Human PDX1 (PDX1 Human)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNSSCPG

   Plasmid

   #183187

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   superfolder GFP
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-MyosinIIC-N-18

   Plasmid

   #55108

   Purpose

   Localization: Cytoskeleton, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

   Insert

   MyosinIIC
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCAP03-act1p

    Plasmid

    #120233

    Purpose

    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing act1p promoter and apramycin resistance gene
    

    Use

    Synthetic Biology

    Mutation

    aac(3)IV (apramycin resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiguide-puro_hORAI1sg

    Plasmid

    #174326

    Purpose

    gRNA targeting hORAI1 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiGuide-Neo-CTRLg1

    Plasmid

    #139450

    Purpose

    Lentiviral vector with non-targeting gRNA and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TT 3xFLAG-KIF20A

    Plasmid

    #192297

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KIF20A

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KIF20A (KIF20A Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ND4 left TALE–G1333 DddAtox C

    Plasmid

    #158092

    Purpose

    expresses the right-side ND4 TALE–DddAtox half in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HA-REDD1 48-232 (#182)

    Plasmid

    #99920

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 48-232

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI.neo-rPAM1-M314I

    Plasmid

    #145802

    Purpose

    expresses catalytically inactive rat PAM-1 in mammalian cells

    Depositor

    Richard Mains, Betty Eipper

    Article

    Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

    Insert

    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    

    Expression

    Mammalian

    Mutation

    changed methionine 314 to isoleucine to inactivat…

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPO685

    Plasmid

    #195300

    Purpose

    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade and native array with PaqCI entry site
    

    Expression

    Bacterial

    Promoter

    Arabinose inducible arapBAD

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. KK501: pMVP (L3-L2) FKBP DD + polyA

    Plasmid

    #121789

    Purpose

    pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FKBP Degron Domain + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. ND4 right TALE–G1333 DddAtox N

    Plasmid

    #158096

    Purpose

    expresses the right-side ND4 TALE–DddAtox half in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. VGFR1_HUMAN_D0

    Plasmid

    #79743

    Purpose

    This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    VGFR1
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only