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pcDNA3-Flag-Trib2

Plasmid

#131155

Purpose

Mammalian expression of flag-tagged mouse TRIB2

Depositor

Warren Pear, Karen Keeshan

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)MBII-85-3'ss mut

Plasmid

#67644

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Use

Tags

Expression

Mammalian

Mutation

3' splice site muattion

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-Abr-HisC

Plasmid

#32509

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR (ABR Human)

Use

Tags

HISC

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mPCSK3 H194L

Plasmid

#122689

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Use

Tags

Expression

Mammalian

Mutation

Changed Histidine 194 to Leucine

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA3-DC+Nzf

Plasmid

#1334

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA3 (Gata3 Mouse)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
GAG-PKB-pcDNA3

Plasmid

#24651

Depositor

Jim Woodgett

Article

Jones et al J Exp Med. 2000 May 15. 191(10):1721-34.

Insert

PKB (AKT1 Human)

Use

Tags

GAG

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA6-rbN1 S77A

Plasmid

#32945

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

SLc9a3r1 (SLC9A3R1 rabbit)

Use

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Change Serine77 to Alanine

Promoter

CMV

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Use

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7ADelta2bp-mCherry

Plasmid

#62638

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
AU1-TTC30B-pcDNA3

Plasmid

#47325

Purpose

Expresses AU1 epitope-tagged TTC30B in mammalian cells

Depositor

Richard Maurer

Article

Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

Insert

TTC30B (Ttc30b Mouse)

Use

Tags

AU1 epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-3XFLAG-m-Lmbr1l

Plasmid

#123593

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

limb region 1 like (Lmbr1l Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-hPXR-FL-T248E

Plasmid

#41617

Depositor

Sridhar Mani

Insert

hPXR-FL-T248E (NR1I2 Human)

Use

Tags

Expression

Mammalian

Mutation

N10D and T248E

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal W427A

Plasmid

#47349

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

W427A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Dazl-myc

Plasmid

#21623

Depositor

David Garbers

Insert

Dazl (Dazl Rat)

Use

Tags

HIS and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RsACR_995-EYFP

Plasmid

#103772

Purpose

Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin RsACR_995

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GcACR_457-EYFP

Plasmid

#103137

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_457

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-TAL-NC2

Plasmid

#43855

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Use

Tags

Flag and FokI nuclease

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Barkor 1-180

Plasmid

#24396

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Contains aa 1-180.

Promoter

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B-HA-TagBFP

Plasmid

#120910

Purpose

Expresses BFP tagged Ptch1-B

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

HA and TagBFP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4.1/HisMax-Syx1

Plasmid

#35708

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Use

Tags

6x HIS and Xpress

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only