pCDNA3-hPXR-FL-T248E
(Plasmid
#41617)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehPXR-FL-T248E
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Alt namePXR
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Alt nameNR1I2
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Alt nameSXR
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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MutationN10D and T248E
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GenBank IDAF061056.1 AAD05436.1
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Entrez GeneNR1I2 (a.k.a. BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWild-type hPXR cDNA acquired from Ronald Evans (Salk Institute, La Jolla, CA)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full length (aa 1-434) human PXR was cloned into pCDNA3. Mutations were made and confirmed by sequencing.
PXR sequence contains N10D mutation when compared to GenBank entry AAD05436.1 (NP_003880.3)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-hPXR-FL-T248E was a gift from Sridhar Mani (Addgene plasmid # 41617 ; http://n2t.net/addgene:41617 ; RRID:Addgene_41617)