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Plasmid#122141PurposeFor making acrosin-GFP-GPI RNADepositorInsertacrosin-GFP-GPI
UseFor in vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z32
Plasmid#120075PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-TagDepositorInsertS-Tag
UsePcr templateTagsS-TagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z30
Plasmid#120073PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-GDepositorInsertVSV-G
UsePcr templateTagsVSV-GExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z27
Plasmid#120070PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 1xHADepositorInsert1xHA
UsePcr templateTags1xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z24
Plasmid#120067PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAPDepositorInsertTAP
UsePcr templateTagsTAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z10
Plasmid#120053PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOkDepositorInsertmKOk
UsePcr templateTagsmKOkExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z01
Plasmid#120044PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P20
Plasmid#120031PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntagDepositorInsert10xSuntag
UsePcr templateTags10xSuntagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P16
Plasmid#120027PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3DepositorInsertDronpa3
UsePcr templateTagsDronpa3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL829
Plasmid#119697Purposeprecursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347CDepositorInsertslow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
UseTagsnoneExpressionBacterialMutationmutations Y283D, G289C and V347CPromoterT5Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Gent
Plasmid#104522PurposeLI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.DepositorInsertApramycin resistance gene
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
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gnu cDNA LD12084
Plasmid#112973PurposeCloning vector carrying the gnu cDNADepositorInsertgnu (gnu Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialMutationPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ePsuACR-mRuby2-ST
Plasmid#109133PurposeAnion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInsertePsuACR-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eiC++-mRuby2-ST
Plasmid#109132PurposeArtificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInserteiC++-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CsChR-mRuby2-ST
Plasmid#109130PurposeCation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertCsChR-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mEGFP
Plasmid#105143PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mEGFP
UseTags81nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCe1885 HC_Kan_Kozak-ATG-MLS_p6
Plasmid#100676Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertKozak-ATG-MLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe2735 HC_Kan_Kozak-ATG-IgkL_p6
Plasmid#100697Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertKozak_ATG_IgkL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-PC-1-CTS
Plasmid#86067PurposeA bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-1 (PKD1 Human)
UseTagsGSTExpressionBacterialMutationcontains the region from 4297-4303PromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD1R
Plasmid#86969PurposeExpresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coliDepositorInsertPCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDHPromoterT7 promoterAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP1298‐1
Plasmid#87248Purposegtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP553‐1
Plasmid#87240Purposegtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)DepositorInsertgtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-Xbp1spliced
Plasmid#80628Purposeexpression of Xbp1 RB isoform in DrosophilaDepositorInsertXbp1 (Xbp1 Fly)
UseTagsExpressionInsectMutationRB isoformPromoterhsp70Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Scmh1-3'UTR
Plasmid#61785PurposeLuciferase reporter containing the 3'UTR of mouse Scmh1DepositorInsertmouse Scmh1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhab-3'UTR
Plasmid#61782PurposeLuciferase reporter containing the 3'UTR of mouse YwhabDepositorInsertmouse Ywhab 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Map3k1-3'UTR
Plasmid#61776PurposeLuciferase reporter containing the 3'UTR of mouse Map3k1DepositorInsertmouse Map3k1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Crtap-3'UTR
Plasmid#61772PurposeLuciferase reporter containing the 3'UTR of mouse CrtapDepositorInsertmouse Crtap 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNH (GB0064)
Plasmid#68165PurposeProvides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid partDepositorInsertSlNH promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPUbc (GB0145)
Plasmid#68170PurposeProvides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid partDepositorInsertSlUbc promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2x35S (GB0222)
Plasmid#68173PurposeProvides the CaMV dual 35S Promoter as a level 0 GoldenBraid partDepositorInsert2x35S promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p6xUAS (GB0179)
Plasmid#68182PurposeProvides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid partDepositorInsert6xUAS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVK_FG
Plasmid#66070PurposeMoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVK_GH
Plasmid#66071PurposeMoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega1R
Plasmid#68240PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2A11 (GB0143)
Plasmid#68189PurposeProvides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid partDepositorInsertSl2A11 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTE8 (GB0144)
Plasmid#68190PurposeProvides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid partDepositorInsertSlE8 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLexAOp (GB0118)
Plasmid#68181PurposeProvides the 4xLexA Operator as a level 0 GoldenBraid partDepositorInsert4xOpLexA
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega2R
Plasmid#68241PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUNC2001
Plasmid#67280PurposeExpresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcwDepositorInsertVibrio cholera photolyase
UseTagsmaltose binding proteinExpressionBacterialMutationnone knownPromoterAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 T180C
Plasmid#66181PurposeExpresses the T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationT180CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C
Plasmid#66180PurposeExpresses the R112C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationR112CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C
Plasmid#66176PurposeExpresses the E105C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationE105CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 Q258A
Plasmid#66724PurposeConstitutive expression of FPR1 Q258A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationQ258APromoterSR-alphaAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Fhod1-3'UTR
Plasmid#61773PurposeLuciferase reporter containing the 3'UTR of mouse Fhod1DepositorInsertmouse Fhod1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Tob1-3'UTR
Plasmid#61774PurposeLuciferase reporter containing the 3'UTR of mouse Tob1DepositorInsertmouse Tob1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Mapk12-3'UTR
Plasmid#61783PurposeLuciferase reporter containing the 3'UTR of mouse Mapk12DepositorInsertmouse Mapk12 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only