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TFORF1201
Plasmid#142751PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSETDB1 (SETDB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA Donor_hEF1a
Plasmid#229779PurposeBxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment markerDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW358f
Plasmid#227935PurposeExpress hIAPP-T7nDepositorInserthIAPP-T7n
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBL066c
Plasmid#227937PurposeExpress Aβ42-GFPDepositorInsertAβ42
UseTagsGFP (F64L, S65T)ExpressionBacterialMutationF64L and S65T in GFPPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-dCasRx
Plasmid#219821PurposeEF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)DepositorInsertdCasRx
UseCRISPRTagsminiTurboExpressionMammalianMutationPromoterEF1a and hU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC2-pA
Plasmid#220754PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC1-pA
Plasmid#220753PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC1
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-pA
Plasmid#220752PurposeExpression of Sun1GFP for nuclei isolationDepositorInsertSun1-GFP-WPRE
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT43
Plasmid#218735PurposeExpresses N-terminally EGFP-tagged IFT43 in mammalian cellsDepositorInsertIFT43 (IFT43 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
WIPI2 sgRNA
Plasmid#207554PurposepX330 expressing Cas9 and a sgRNA targeting the WIPI2 locusDepositorInsertCGCGCGCCCAGCCATGAACC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HR1b-C (B3-B4)
Plasmid#211851PurposeCDS for HR1b degron sequence in domestication vectorDepositorInsertHR1b
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-ΔPS
Plasmid#214901Purposeexpression of the truncated variants of the SHIP2 without phosphatase domainDepositorInserthuman SHIP2 delta aa 419-739 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 419-739aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP
Plasmid#214367PurposeExpression of the Blue Fluorescent ProteinDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB_syb25
Plasmid#197992PurposeExpressed sybody 25 as a C-terminal myc-His-tagged constructDepositorInsertSybody 25
UseTagsMyc-HisExpressionBacterialMutationPromoterArabinoseAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1508
Plasmid#143817PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMTF1 (MTF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xnls-G-Ca-FLITS
Plasmid#191463PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTags3xNLSExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-EGFP-RhoA-T19N
Plasmid#12967DepositorInsertRhoA DN (RHOA Human)
UseTagsEGFPExpressionMammalianMutationT19N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Po-ER Long P2A
Plasmid#164113PurposeDetect the long Peroxisomes-Endoplasmic reticulum contactDepositorInsertSplit YFP Po-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-3xFLAG
Plasmid#69837PurposeThis plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-eGFP-Puro
Plasmid#79823PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-CLIP170-N-18
Plasmid#54044PurposeLocalization: Microtubule Binding , Excitation: 487, Emission: 509DepositorInsertCLIP170 (CLIP1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-C1
Plasmid#54611PurposeLocalization: C1 Cloning Vector, Excitation: 558, Emission: 583DepositorTypeEmpty backboneUseTagsDsRed2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA flag PPAR gamma
Plasmid#8895PurposeMammalian expression of flag-tagged PPAR gammaDepositorInsertPPAR gamma (Pparg Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7f
Plasmid#104483PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7f
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF6-CSPG4-myc-his
Plasmid#69037PurposeExpression of CSPG4 in mammalian cellsDepositorInsertCSPG4 (CSPG4 Human)
UseTagsHis and MycExpressionMammalianMutationnonePromoterEF-1 alphaAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAX2
Plasmid#117398Purposeallelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switchDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos4b-N1
Plasmid#54814PurposeLocalization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneUseTagsmEos4bExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Dendra2-Golgi-7
Plasmid#57722PurposeLocalization: Golgi, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertGolgi (B4GALT1 Human)
UseTagsDendra2ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBabe-c-myc-zeo
Plasmid#17758DepositorInsertc-myc (MYC Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
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GFP-Ub KO.G76V
Plasmid#11932DepositorInsertUbiquitin KO.G76V (RBX1 Human)
UseTagsGFPExpressionMammalianMutationConjugation deficient mutant. All 7 lysines of ub…PromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
TFORF3058
Plasmid#144534PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEBF1 (EBF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-GFP-MYH9
Plasmid#168273PurposeExpresses MYH9 in mammalian cellsDepositorInsertMYH9 (MYH9 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCW57.1_V5_TurboID_FLAG_Ct
Plasmid#194073Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-TurboID-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_Halo_GluA2
Plasmid#170454PurposeExpresses halo tagged GluA2 in neuronal cellsDepositorInsertGluA2 (Gria2 Mouse)
UseLentiviralTagsHaloExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Kv7.1/pcDNA3.1
Plasmid#111452PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)
UseTags8xHis and EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS1
Plasmid#196977PurposeExpresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS1 tag
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Hygro
Plasmid#164142PurposeRetroviral expression vector with hygromycin resistanceDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFG2
Plasmid#87250Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-NatDepositorInsertNat
UseTagsparS segmentsExpressionYeastMutationPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pN1/G3BP1-iRFP
Plasmid#129339PurposeStress granule markerDepositorInsertiRFP (from Dr. M. Matsuda)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[21-881]-C-int
Plasmid#199202PurposeGateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expressionDepositorInsertsplit-intein-Gal4[21-881]-C-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE-Plus
Plasmid#214079PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-pBAD
Plasmid#54800PurposeLocalization: Protein Expression Vector , Excitation: 604, Emission: 659DepositorTypeEmpty backboneUseTags6xHis and mCardinalExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits
