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Plasmid#228976PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-8
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-9
Plasmid#228977PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-9
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225649PurposetetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA, araBAD and LacO3O1 in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromotertetA, araBAD and LacO3O1 promotersAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xCBX7CD_W35A-IFP2.0C
Plasmid#215755PurposeExpresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensorsDepositorInsert2xCBX7CD_W35A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM 1996
Plasmid#217825PurposeExpresses putative photoreceptor SA-phr1 from S. alba. This gene shows high homology to the phr genes in prokaryotes.DepositorInsertSA-phr1
UseTagsMaltose-binding proteinExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GCaMP6f-WPRE
Plasmid#216275PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6fDepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman Synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FAS-GCaMP6f-WPRE
Plasmid#216277PurposeAAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman synapsin 1 (hSyn)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-GCaMP6f-WPRE
Plasmid#141237PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterHuman elongation factor-1 alpha (EF-1 alpha)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD_W42A-IFP2.0C
Plasmid#215751PurposeExpresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD_W42A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2x3APWWP_K299E_D333A-IFP2.0C
Plasmid#215753PurposeExpresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP_K299E_D333A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45
Plasmid#208796PurposeXenopus laevis FLAG5-His10-TEV-Cdc45DepositorInsertCdc45 (cdc45.L Frog)
UseTagsFLAG and HisExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-WM
Plasmid#208798PurposeXenopus laevis His6-Myc-DONSON (W234L, M463T)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationW234L; M463TPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-S437A
Plasmid#208803PurposeXenopus laevis His6-Myc-DONSON (S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationS437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-ΔD1
Plasmid#208804PurposeXenopus laevis His6-DONSON (1-155aa removed)DepositorInsertDONSON (donson.L Frog)
UseTagsHisExpressionBacterialMutationΔD1, deletion of 1-155aaPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7 (2-180)
Plasmid#190066PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEVExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7-Strep (2-180)
Plasmid#190067PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEV and StrepExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BBLF2/3
Plasmid#195831PurposeGateway Entry Clone of Epstein-Barr virus BBLF2/3DepositorInsertBBLF2/3 (BBLF2/BBLF3 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BcRF1(vTBP)
Plasmid#195832PurposeGateway Entry Clone of Epstein-Barr virus BcRF1DepositorInsertBcRF1 (BcRF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BCRF1(vIL10)
Plasmid#195777PurposeGateway Entry Clone of Epstein-Barr virus BCRF1, vIL-10DepositorInsertBCRF1, vIL-10 (BCRF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BaRF1(RNR-s)
Plasmid#195795PurposeGateway Entry Clone of Epstein-Barr virus BaRF1DepositorInsertBaRF1 (BaRF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only