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pCW-GFP-CtIP

Plasmid

#71109

Purpose

mammalian expession of GFP-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-MAPKAPK2

Plasmid

#82720

Purpose

Expresses human MAPKAPK2

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MAPKAPK2 (MAPKAPK2 Human)

Use

Tags

3xFLAG and GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pDONR223_ETS1_WT

Plasmid

#82118

Purpose

Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ETS1 (ETS1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-hERalpha

Plasmid

#101141

Purpose

Mammalian expression of full-length human estrogen receptor-alpha

Depositor

Elizabeth Wilson

Article

Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

Insert

estrogen receptor-alpha (ESR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Phusion

Plasmid

#206210

Purpose

Inducible expression of DNA polymerase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

Phusion DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p3HA-hMIB1

Plasmid

#33317

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB1 (MIB1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Use

Tags

C-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Use

Tags

mVenus

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Neo (657-2)

Plasmid

#17447

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHR CD22-Megf10 CAR

Plasmid

#113016

Purpose

for production of lentivirus encoding CD22 CAR-P Megf10

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD22 CAR-P Megf10 intracellular domain

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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phage UbiC G3BP1-GFP-GFP

Plasmid

#119950

Purpose

Lentiviral vector expressing G3BP1-GFP fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-CAG-AtAFB2.F74A-IRES-puro

Plasmid

#216245

Purpose

Express AtAFB2.F74A mutant without tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

AtAFB2.F74A

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

Expression

Mammalian

Mutation

F74A mutation

Promoter

CAG

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE3

Plasmid

#90337

Purpose

AHR - Aryl hydrocarbon receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

AHR

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF3363

Plasmid

#144839

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR5A1 (NR5A1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA E545K

Plasmid

#12525

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA E545K (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

E545K. Tumor-derived allele.

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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Drp1-EGFP-StrepII

Plasmid

#174429

Purpose

Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

EGFP-StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSBtet-BP

Plasmid

#60496

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

Plasmid

#106171

Purpose

cre mediated red to green shift

Depositor

Ewa Snaar-Jagalska

Article

Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

Inserts

dsRED-express2
eGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLH

Plasmid

#201671

Purpose

Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

Insert

Polymerase eta (POLH Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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ABE8r-V106W

Plasmid

#208295

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-V106W-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTrEno

Plasmid

#115474

Purpose

Eno-promoter driven expression vector for protein production in Trichoderma reesei

Depositor

Steve Decker

Article

Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

Insert

Cel7A

Use

Unspecified

Tags

Expression

Mutation

Introns have been removed

Promoter

Trichoderma reesei Enolase (Eno) promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRE57-Ter

Plasmid

#170635

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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mScarletI-Rab7

Plasmid

#112955

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Rab7

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP670nano

Plasmid

#127434

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

LifeAct-miRFP670nano

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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p201N Cas9

Plasmid

#59175

Purpose

Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
nptII

Use

CRISPR

Tags

Expression

Mutation

Promoter

2x35S and StUbi-3P

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGreenLantern

Plasmid

#161912

Purpose

mGreenLantern fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-LaminB_C1

Plasmid

#189776

Purpose

Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-LaminB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-MED1LCD

Plasmid

#204404

Purpose

E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

MED1(947-1581) (MED1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABE8e

Plasmid

#138502

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9 (KKH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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