Addgene

We narrowed to 4,919 results for: pcDNA3.1+

Showing: 3121 - 3140 of 4919 results

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pcDNA flag PPAR gamma

Plasmid

#8895

Purpose

Mammalian expression of flag-tagged PPAR gamma

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-CalfluxCTN

Plasmid

#190068

Purpose

Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

Depositor

Carl Johnson

Article

Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

Insert

CalfluxCTN

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-NTEVp(WT)

Plasmid

#137831

Purpose

Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-NTEVp(WT)

Use

Tags

3x FLAG and NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-CTEVp(WT)

Plasmid

#137832

Purpose

Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CTEVp(WT)

Use

Tags

3x FLAG and NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Use

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670nano3

Plasmid

#184666

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-human alpha-tubulin

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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hHSF1 K80R

Plasmid

#118350

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to R.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80R

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pC0078 RESCUE

Plasmid

#130661

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd (RESCUE)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFH2.2_mCherry-MS2x12

Plasmid

#205521

Purpose

Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-PANX1(HCVp)

Plasmid

#87698

Purpose

Express HCV protease-activatible pannexin-1 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PANX(HCVp)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDN77

Plasmid

#61347

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS2 and NES IkBa

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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MaLionB

Plasmid

#113910

Purpose

Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHLuc Control

Plasmid

#141091

Purpose

pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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Tailless VinculinTS

Plasmid

#26020

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

tailless vinculinTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1

Plasmid

#1026

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pEV53D

Plasmid

#44168

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Equine infectious anemia virus (EIAV) gag-pol,rev, and S2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

A131T mutation in gag; Deletion of viral LTR sequ…

Promoter

CMV

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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hSOD1-FLAG

Plasmid

#182922

Purpose

Mammalian expression plasmid for human WT SOD1

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

[Cu-Zn] Superoxide dismutase 1 (SOD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Insert

Src biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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beta-arrestin2 mYFP

Plasmid

#36917

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin2 (Arrb2 Rat)

Use

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pDV10_pCMV-ADAR1-p150

Plasmid

#200405

Purpose

Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

ADAR-p150

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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