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pEGFPC1-Intu

Plasmid

#65430

Purpose

For mammalian expression of EGFP-mouse Inturned

Depositor

Aimin Liu

Article

Zeng et al Dev Biol. 2010 Mar 15;339(2):418-28. doi

Insert

Inturned (Intu Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C-t

Plasmid

#89981

Purpose

Expresses the GFP-tagged C-terminus of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-182

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJUMP41-2A(sfGFP)

Plasmid

#126985

Purpose

Level 2 vector. sfGFP reporter. Origin R6K (pir conditional).

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Jpx9

Plasmid

#120379

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 mitoAURKA

Plasmid

#157754

Purpose

Expression of AURKA:GFP targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

AURKA without the first 30 amino acids replaced b…

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPNT6-eGFP

Plasmid

#71303

Purpose

This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression

Depositor

Gian Luca Forni, Marco Musso

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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Nsp14D-EGFP

Plasmid

#165125

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14D (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C1-GFP

Plasmid

#46041

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2006 Aug 25;281(34):24662-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1 (Myh14 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/GFPZNF598-9P-9A

Plasmid

#141192

Purpose

Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

all 3 proline repeats are mutated to Alanine (9xP…

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-TSAd-wt

Plasmid

#84562

Purpose

mammalian expression of TSAd-EGFP fusion

Depositor

Anne Spurkland

Insert

TSAd (SH2D2A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.sfGFP-RepEntry.iPAC

Plasmid

#100893

Purpose

Lentiviral CRISPR-Cas9 Reporter

Depositor

Dirk Heckl

Article

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Inserts

sfGFP
PAC

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-d2eGFP-20

Plasmid

#21970

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

CMV d2eGFP miR-20 sponge

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only
pGFAP::MyrGFP_v2

Plasmid

#160277

Purpose

Expresses GFP from the Gfa2 promoter. This plasmid is a new version of Plasmid 22672 and includes a stop codon in the myrEGFP insert.

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

myrGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Gfa2

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pClneoEGFPC RSF-1

Plasmid

#66862

Purpose

Express GFP-fused C.elegans RSF-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

RSF-1 (rsf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

S18G, S229T, I403T and F563S mutations in RSF-1 c…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pmCD8-GFP,y+

Plasmid

#24350

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

Yellow gene

Use

Tags

CD8-GFP

Expression

Insect

Mutation

Promoter

Available since

April 12, 2010

Availability

Academic Institutions and Nonprofits only

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pBEAST2-sfGFP

Plasmid

#126575

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ oDam_f_GFPoNLS

Plasmid

#85819

Purpose

iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

oDam_f_GFPoNLS

Use

Tags

oNLS (optimized nuclear localization signal) C te…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Promoter

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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iLID-eGFP-C2

Plasmid

#114415

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pABCb10aa1-70-GFP

Plasmid

#19038

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

amino acids 1 - 70 of the targeting sequence of ABCb1

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-B2UP49_GFP

Plasmid

#103071

Purpose

Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))

Use

CRISPR

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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