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Plasmid#186834PurposeExpresses FLAG-BMAL1-S519A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 519 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-S1pr1-R120A
Plasmid#203707PurposeOverexpression of FLAG-S1PR1-R120A mutant.DepositorInsertFlag-S1PR1-R120A (S1PR1 Human)
UseTagsFlagExpressionMammalianMutationR120APromoterAvailable sinceDec. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MTUS1 zeo
Plasmid#192323PurposeLentiviral expression vector for an inducible 3xFLAG-MTUS1DepositorInsertMTUS1 (MTUS1 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAH1 (lenti hLPL-FLAG)
Plasmid#102448PurposeFLAG-tagged human LPL in lentiviral vectorDepositorInsertLipoprotein lipase (LPL Human)
UseLentiviralTags6x His and FLAGExpressionMutationPromoterCMVAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-LLAA(20)
Plasmid#124397PurposeFull-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
UseTagsFlagExpressionMammalianMutationL897A/L900A mutations (full length protein is 213…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-YTHDF2
Plasmid#38089DepositorInsertYTHDF2 YTH domain family, member 2 (YTHDF2 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-MYEF2
Plasmid#38069DepositorInsertMYEF2 myelin expression factor 2 (MYEF2 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mCherry-FLAG:cat
Plasmid#211831PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.DepositorInsertmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
CDK4mut(K35M)-3XFlag-pBabe
Plasmid#73559Purposevector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tagsDepositorInsertCDK4mut(K35M)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterSV40Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-dSpCas9-HF1
Plasmid#92115PurposeExpression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)DepositorInsertdead/inactive SpCas9-HF1 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterCbhAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-Hygro-3xFLAG
Plasmid#185359PurposeVector backbone for mammalian expression of proteins with an N-terminal 3xFLAG tag.DepositorInsert3xFLAG
UseTags3xFLAGExpressionMammalianMutationWTPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-S320A
Plasmid#112491PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationS320APromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-S305A-3xFLAG
Plasmid#69839PurposeThis plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationS305APromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-S155,172D
Plasmid#74442PurposeLentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseLentiviralTagsFlagExpressionMammalianMutationSer 155 Asp, Ser 172 Asp (Numbering based on MFF …PromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-DDB1 (1140H)
Plasmid#174849PurposeExpression of DDB1DepositorInsertDDB1 (DDB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S277G
Plasmid#13615DepositorUseTagsFLAGExpressionMammalianMutationChanged catalytic Serine 277 to AlaninePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-LRRC55-V5-Flag-His
Plasmid#127819PurposeExpression of human LRRC55 in mammalian cellsDepositorInsertLRRC55 (LRRC55 Human)
UseTagsV5, FLAG, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-flag-hDDI2_pET11a
Plasmid#169121Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only