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eGFP-pk-H302A

Plasmid

#115618

Purpose

Expresses human Parkin H302A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H302A

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-hSyntenin2b

Plasmid

#89437

Purpose

mammalian expression of EGFP tagged human Syntenin2b

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

*see below

Promoter

CMV

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G05 sfGFP

Plasmid

#67045

Purpose

Cell-free expression control

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

sfGFP

Use

Cell-free expression

Tags

mCherry

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Cx26-sfGFP

Plasmid

#69026

Purpose

Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx26 (Gjb2 Rat)

Use

Tags

non-monomerized superfolder GFP (V206)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Jpx9C104A

Plasmid

#120380

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

C321A

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCX4-EGFP

Plasmid

#36120

Depositor

Larry Goldstein

Article

Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

Insert

eGFP (eGFP )

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
LOC8R-PAGFP

Plasmid

#66898

Purpose

PAGFP targeted to mitochondria using the synthetic LOC8R presequence

Depositor

Georg Ramm

Insert

LOC8R-PAGFP

Use

Tags

LOC8R

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTK52_GFP-NuMA-C-3A

Plasmid

#46350

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA C-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

C-terminal fragment from 1700-2115, and changed T…

Promoter

pBABE

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pGH312_wtGFP Q80H

Plasmid

#85409

Purpose

lentiviral expression vector for wtGFP Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP Q80H

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-eGFP

Plasmid

#136098

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_eGFP

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12_eGFP-linker

Plasmid

#136086

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_eGFP(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPTK017-3b-yEGFP_Sec

Plasmid

#84976

Purpose

Type 3b, Green fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe_PepTSo

Plasmid

#58334

Purpose

Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

So Iwata, Simon Newstead

Article

Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

Insert

PepTso

Use

Tags

GFP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pS23·Pm-GFP

Plasmid

#122593

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pm

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shHmga2#3

Plasmid

#122291

Purpose

Knockdown for mouse Hmga2

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for mouse Hmga2 (Hmga2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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CHL1 wt-eGFP

Plasmid

#117876

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

CHL1 wt-eGFP (AT5G40090 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-i5-msfGFP

Plasmid

#180332

Purpose

mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v7 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1-i5

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i5-msfGFP

Plasmid

#180327

Purpose

mammalian expression of human SEPT9_i5 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v7 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Tac-TC

Plasmid

#162492

Purpose

Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

partial length Tac (TMD and cytosolic tail) (IL2RA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Tac is in partial length with its TMD and cysotol…

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBW412hrpL-cIgfp

Plasmid

#61438

Purpose

pSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs32-CI-LVA-t-plam-30gfp-t

Use

Tags

LVA

Expression

Bacterial

Mutation

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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