-
Plasmid#11642PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-tdTomato
Plasmid#99116PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomatoDepositorInsertDIO-tdTomato
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
ReNL/pcDNA3
Plasmid#85203PurposeRed color variant of bright luminescent proteinDepositorInsertRed enhanced Nano-lantern
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
UseTagsExpressionBacterialMutationPromoterPPSPAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-mCherry
Plasmid#174188PurposeExpress mCherry with mitochondria-targeting sequence in mammalian cellsDepositorInsertmCherry with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-jGCaMP7b-XN
Plasmid#178362PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.DepositorInsertjGCaMP7b-XN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
negMaLionR
Plasmid#113909Purposenegative control for Red ATP sensorDepositorInsertnegMaLionR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NG-PE2-BSD
Plasmid#176933PurposeExpresses PE2-NG variant in mammalian cellsDepositorInsertPE2-NG variant
UseLentiviralTagsSV40 NLSExpressionMammalianMutationPromoterEFSAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
Plasmid#64218PurposeExpression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-BFP-P2A-E1BExpressionMammalianMutationPromoterCBh and U6Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP YAP1
Plasmid#193675PurposeConstitutive lentiviral expression of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT1T2
Plasmid#50593PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, gRNA scaffold, OsU3t plus, TaU3p, T2
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
FlucSM-HA-GFP11-N1
Plasmid#91955PurposeExpresses aggregation-prone FlucSM-HA-GFP11 in mammalian cellsDepositorInsertFlucSM-HA-GFP11
UseTagsExpressionMammalianMutationR188QPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVβ-p300.DY-myc
Plasmid#30490DepositorInsertp300 (EP300 Human)
UseTagsMycExpressionMammalianMutationaspartic acid 1399 converted to tyrosine (Acetyla…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFFLuc-IVT
Plasmid#205147PurposeIVT template of FireFly luciferase stop codon between the ORF and MCS and polyADepositorInsertFireFly luciferase
UseLuciferase; In-vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Peredox
Plasmid#73807PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)DepositorInsertPeredox
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-mRFP1
Plasmid#53745PurposeContains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vectorDepositorInserthistone H2B (H2BC21 Human)
UseTagsmRFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-GEMSgRNA2
Plasmid#204768PurposepU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.DepositorInsertguide RNA targeting GEMS m6A sensor mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF
Plasmid#82021PurposecLac143; pALM143; IPTG inducible systemDepositorInsertYFP
UseTagsExpressionMutationPromoterPcptoo_C38T,A39TAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-300(HAT-)
Plasmid#23254DepositorInsertp300 (EP300 Human)
UseTagsExpressionMammalianMutationH1415A E1423A Y1424A L1428S Y1430A H1434APromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only