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Plasmid#47645PurposepAC-EsaR with a c659t mutation in esaRDepositorInsertesaR-V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Valine 220 to AlaninePromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH034
Plasmid#42150DepositorInsertRT-cassette
UseTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092709
Plasmid#153859PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationNearly native encoding;handful SNPs as watermarks…PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbA5c-RFP
Plasmid#35281DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGWB701NL3F10H
Plasmid#141288PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector
Plasmid#172891PurposeContains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites
UseTagsExpressionBacterialMutationPromoterProD, nTETO, and pBAD /AraCAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKS1
Plasmid#24636DepositorInsert'tesA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJL005
Plasmid#92327PurposeExpress FtsZ tagged with mEos3.2 in E coliDepositorInsertFtsZ
UseTagsmEos3.2ExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBL7026
Plasmid#128811PurposeFluoride riboswitch-regulated 3-way junction dimeric broccoliDepositorInsertFluoride riboswitch-regulated 3-way junction dimeric broccoli
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB8c-GFP
Plasmid#35362DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
RAD Module G8-C1
Plasmid#38208DepositorInsertsSET Integrase
Bxb1 Excisonase
RESET Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGWB401NL3F10H
Plasmid#141285PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPYC0CM0257
Plasmid#162308PurposeLevel 0 C-terminal tag part for MoClo assembly, TTCG-GCTTDepositorInsertC-terminal tag, HiBiT-stop, frame2 (TTC-G)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0281
Plasmid#162302PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, [S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah2
Plasmid#122569PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-ccdB
Plasmid#99915PurposeBasic cloning vector for the expression of imaging-based optimization sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbA1c-RFP
Plasmid#35335DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTrcAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST1K18ms
Plasmid#72646PurposeGateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringaeDepositorTypeEmpty backboneUseSucrose counter-selection allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceMarch 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
AEB-F12
Plasmid#63849PurposeThis pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene.DepositorInsertF12 fluoride riboswitch
UseSynthetic BiologyTagsExpressionMutationPromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only