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Plasmid#64112PurposeExpression of St1 dCas9-3XGFP in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XsfGFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-P4M-SidM
Plasmid#51469PurposeBiosensor for PtdIns4PDepositorInsertP4M-SidM(546-647)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Rac1-2G
Plasmid#66110PurposeSecond generation Rac1 FRET biosensorDepositorInsertSecond generation Rac1 FRET biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
mito-PAGFP
Plasmid#23348PurposeExpresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.DepositorInsertPAGFP
UseTagsmitoExpressionMammalianMutationPromoterAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pY109 (lenti-LbCpf1)
Plasmid#84740PurposeLenti virus delivery of LbCpf1 and crRNA guideDepositorInsertshuLbCpf1
puromycin resistance gene
UseLentiviralTags3xHA, NLS, and P2AExpressionMutationPromoterEFSAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
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HA-mDyn1 pcDNA3
Plasmid#36263DepositorInsertDynamin 1 (Dnm1 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLgw-MBD3-V5-Dam
Plasmid#55605PurposeExpression of Dam fused MBD3 in mammalian cellsDepositorInsertMBD3 (MBD3 Human)
UseLentiviralTagsDamID (DNA adenine methyltransferase identificati…ExpressionMammalianMutationPromoterHSPAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediateTagsExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF.CMV.RFP
Plasmid#17619DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCGN-MEF2D
Plasmid#32963PurposeFor mammalian expression of mouse MEF2D with an HA tagDepositorInsertMEF2D (Mef2d Mouse)
UseTagsHAExpressionMammalianMutationE287GPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-EGFP
Plasmid#73461PurposeExpresses EGFP for monosynaptic circuit mappingDepositorInsertEGFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
MLRV2: NF-kb-SMAD-DBE-P53-AP1
Plasmid#124536PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1DepositorInserts5 copies of the NF-kb DNA binding motif
7xSMAD_RE::FLuc
3xDBE_BE::Renilla (FOXO)
2xP53_RE::NLuc
6xAP-1_RE::GrRenilla
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-PR50
Plasmid#84905Purposeinduce expression of dipeptide repeat PR in yeastDepositorInsertPR50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP HA
Plasmid#59759Purposeencodes multiple copies of an epitope tag HADepositorInsertsmFP _HA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5
Plasmid#64613Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseLentiviralTagsV5ExpressionMutationS33A, S37A, T41A, S45APromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 - hSRSF6
Plasmid#120419Purposeoverexpression of human SRSF6DepositorInsertSRSF6 (SRSF6 Human)
UseTagsExpressionMammalianMutationPromoterCMV immediate/earlyAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
ER-mNeonGreen
Plasmid#137804PurposeVisualization of the Endoplasmic ReticulumDepositorArticleInsertKDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696Purposeallows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only