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Plasmid#174140PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…PromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-Stuffer-HepmTurquoise2
Plasmid#192826PurposeContains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoterDepositorInsertmTurquoise2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHepatocyte-specific promoter (HS-CRM8-TTRmin modu…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH183
Plasmid#122856PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-scribP305L
Plasmid#24601DepositorInsertScribble (SCRIB Human)
UseRetroviralTagsExpressionMammalianMutationP305LPromoterAvailable sinceMay 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-PvanCC-mVenus_vanR
Plasmid#137664PurposeConjugative vector derived from pSHDY. Constitutive expression of the repressor vanR, as well as vanillate-inducible expression of mVenus.DepositorInsertPvanCC:mVenus
UseTagsExpressionBacterialMutationPromoterPvanCCAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2B
Plasmid#118228PurposeEmpty E. coli - B. burgdorferi shuttle vector, blasticidin S resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
C413-E15: attB4-EF1p>-attB1r
Plasmid#162920PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman EF1alpha promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab9aQ66L
Plasmid#169072PurposeExpress human constitutively active form of Rab9a(Q66L) in mammalian cellsDepositorInsertRab9a (RAB9A Human)
UseTagsacGFP1ExpressionMammalianMutationQ66LPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG.3xHalo-GFP-SPC25
Plasmid#176206PurposeKinetochore anchor for TMP-Halo dimerization systemDepositorInsert3xHalo-GFP-SPC25
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald
Plasmid#203802PurposeLentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationT23NPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Talin Head
Plasmid#186140PurposeFor expression of recombinant Talin head domain in bacteriaDepositorInsertTLN1 (TLN1 Human)
UseTagsHis6 (tev cleavage)ExpressionBacterialMutationPromoterLacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-RCC1-polyA
Plasmid#59762PurposeExpress Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRCC1
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-eGFP-RAB11
Plasmid#203731PurposeAAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoterDepositorInsertRAB11 (RAB11A Human)
UseAAVTagseGFPExpressionMammalianMutationPromoterHuman synapsin promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
UseTagsExpressionBacterialMutationN/APromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5C6A,P-J23106-geneIII
Plasmid#80860PurposePhagemid (encodes evolved cI5C6A,P and M13 gene III)DepositorInsertsM13 gene III
cI5C6A,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pML3009
Plasmid#193256PurposeGamma-aminobutyric acid sensor expressing eYFP reporterDepositorInsertsgabR
eYFP
UseSynthetic BiologyTagsExpressionMutationPromotersynthetic promoter P2 and synthetic promoter PGab…Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPS0359
Plasmid#115508PurposeReporter Plasmid. Taurine-inducible expression of GFPDepositorInsertsfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterTaurine-inducible promoter of S. meliloti Promote…Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rap2b
Plasmid#118320PurposeExpression of human Rap2bDepositorInsertRap2b (RAP2B Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only