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pcDNA3-sACE2v5-Fc(IgG1)

Plasmid

#145168

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 5

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE2

Plasmid

#120396

Purpose

Expresses HF2-BE2 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE2

Use

Tags

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL23

Plasmid

#135104

Purpose

Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL23 codon optimized

Use

Tags

HA-Strep-Strep (N terminal on backbone)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL23-CStHA

Plasmid

#135099

Purpose

Codon-optimized HSV-1 UL23 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL23 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas8e

Plasmid

#106270

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasA

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCse2

Plasmid

#106271

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasB

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas7

Plasmid

#106272

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasC

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas5

Plasmid

#106273

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasD

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K5-9R

Plasmid

#63562

Purpose

Expression of human H2A with K5R and K9R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 5 and Lysine 9 are now Arginine

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA HA FAK Y576F

Plasmid

#50506

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Use

Tags

HA

Expression

Mammalian

Mutation

Y576F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 L198A

Plasmid

#8975

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

L198A

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 AK3 Hyg

Plasmid

#37539

Depositor

David Sidransky

Article

Chang et al PLoS One. 2011;6(6):e20806. Epub 2011 Ju

Insert

AK3 (AK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/NS5-Af-mut

Plasmid

#120829

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

ZIKV NS5 V114M/V872M - "african mut"

Use

Tags

V5

Expression

Mammalian

Mutation

V114M/V872M

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ZifCV1.49

Plasmid

#59009

Purpose

Mitochondrial Zn-binding FRET biosensor

Depositor

Amy Palmer

Article

Park et al ACS Chem Biol. 2012 Aug 10.

Insert

mito-ZifCV1.49 genetically encoded Zn(II) sensor

Use

Tags

See comment

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 HRPT2 L64P

Plasmid

#11052

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 L64P (CDC73 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

L64P

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
Kohinoor-Zyxin/pcDNA3

Plasmid

#67777

Purpose

Zyxin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Zyxin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-RG-mycStrep

Plasmid

#31840

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R95G

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
HA-PKB-pcDNA3-K179A

Plasmid

#24652

Depositor

Jim Woodgett

Insert

PKB (AKT1 )

Use

Tags

HA

Expression

Mammalian

Mutation

K179A

Promoter

Available since

June 1, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA3.1 Bach1 S990A

Plasmid

#73643

Purpose

Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).

Depositor

Sharon Cantor

Insert

Bach1 (BACH1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

S990A (BRCA binding mutant)

Promoter

CMV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA-mito-ZifCV1.173

Plasmid

#59010

Purpose

Mitochondrial Zn-binding FRET biosensor

Depositor

Amy Palmer

Article

Park et al ACS Chem Biol. 2012 Aug 10.

Insert

mito-ZifCV1.173 genetically encoded Zn(II) sensor

Use

Tags

See comment

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only