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pGKO2-Gateway

Plasmid

#63618

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Tags

Expression

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMEXC3H

Plasmid

#110080

Purpose

Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSBDEST.IP

Plasmid

#79472

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, IRES Puromycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Tags

Expression

Mutation

Promoter

none

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAVA

Plasmid

#127464

Purpose

AVA-seq plasmid with duel BstXI sites

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSJ111

Plasmid

#78707

Purpose

Modular vector for constitutive secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pveg

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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TroponinT-Gateway

Plasmid

#46026

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pBbB6c-GFP

Plasmid

#35356

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

LlacO-1

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pGL0_16 [2μ ORI]

Plasmid

#198929

Purpose

Level 0 part

Depositor

Nili Ostrov, Charlie Gilbert, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

2μ ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMEXNH3

Plasmid

#110079

Purpose

Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37

Plasmid

#196657

Purpose

Plasmid for overexpression of native initiator tRNA (A37)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37 expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

metY native promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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CDS_aadA_chlo

Plasmid

#163935

Purpose

L0 part - CDS

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

aadA gene for spectinomycin resistance

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMM0531

Plasmid

#128988

Purpose

Part plasmid for VP16-CIB1, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-VP16-CIB1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0530

Plasmid

#128987

Purpose

Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-ZiF268DBD-CRY2PHR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0528

Plasmid

#128985

Purpose

Part plasmid for pZF(3BS), Type 2 (promoter) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BS)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-GW

Plasmid

#63780

Purpose

yeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insert

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Yeast

Mutation

Promoter

GPD

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pACYC-PLlambda-alkSm4-3

Plasmid

#44383

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkSm4-3

Use

Synthetic Biology

Tags

Expression

Mutation

Q410I, S470T, K546E, E775D

Promoter

PLlambda

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pGL0_13 [pIP501 ORI]

Plasmid

#198927

Purpose

Level 0 part

Depositor

Nili Ostrov, Charlie Gilbert, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Mutation

Promoter

polH (polyhedrin)

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

Plasmid

#183077

Purpose

Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and crRNA ending with a HDV ribozyme

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFRETvr-2in1-CC

Plasmid

#105117

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mVenus/tagRFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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