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Showing: 3081 - 3100 of 3879 results

  4. pLenti GW V5 Eco/Dam hum LaminA

    Plasmid
    #182673
    Purpose
    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.
    Depositor
    Bas van Steensel
    Article
    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:
    Insert
    Lamin A (LMNA Human)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Mutation
    Promoter
    HSP
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182673-plasmid-map-sequence-id-357405

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  7. pSLIK_Hyg_mTurquoise2_Atg4BC74A

    Plasmid
    #161733
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Promoter
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161733-plasmid-map-sequence-id-318425

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  10. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid
    #111147
    Purpose
    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    actin gamma 1 (ACTG1 Human)
    Use
    Pichia pastoris integration vector
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    codon-optimised for expression in Pichia pastoris
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111147-plasmid-map-sequence-id-214068

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  13. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Promoter
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pD649-HAsp-IL1R2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156769
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL1R2 (IL1R2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156769-plasmid-map-sequence-id-382023

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  18. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid
    #137722
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Truncated at amino acid 1328
    Promoter
    Tight TRE promoter
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137722-plasmid-map-sequence-id-352678

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Showing: 3081 - 3100 of 3879 results