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Plasmid#47951PurposertTA positive feedback loop with single tet operator in the GAL1 promoterDepositorInsertrtTA
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE E171Q HDEL
Plasmid#32124DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, E171QPromoterAvailable sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE K108A GFP
Plasmid#32126DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, K108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE K108A HDEL
Plasmid#32122DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, K108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
Plasmid#69100PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFPDepositorInsertsyZ3EV cassette
Z3 promoter
yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ379
Plasmid#74215PurposeCombined sgRNA/Cas9 vector with NatMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-Opi1 + terminator
Plasmid#58836PurposeExpresses GFP-Tagged Opi1 + Terminator in yeastDepositorInsertOpi1p (OPI1 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS415-IVa
Plasmid#31460DepositorInsertlinker
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMZ377
Plasmid#74214PurposeCombined sgRNA/Cas9 vector with LEU2 markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-SRM (sortase recognition motif)-hphMX4
Plasmid#54488PurposePCR based C-terminal SRM tagging in yeastDepositorInsertSRM (sortase-recognition motif)
UseYeast genome targetingTagshphMX4ExpressionMutationPromoterAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRR1
Plasmid#166096PurposeGal inducible expression of Cas9 WTDepositorInsertWT spCas9
UseCRISPRTagsExpressionYeastMutationPromoterGal-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMZ376
Plasmid#74213PurposeCombined sgRNA/Cas9 vector with KanMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC6-TEFkFLP(LYS2)
Plasmid#49458Purposeoverespression of Flp step-arrest mutant in yeast, LYS2 selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterTEF1Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPICZa-STE2-1D4
Plasmid#133436PurposeMutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionBacterialMutationC59S/C252APromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCA/GAL4(848).ER
Plasmid#108215PurposeYeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor aDepositorInsertGal4-ERalpha HBD
UseTagsExpressionYeastMutationERalpha HBD has G400V mutationPromoterADH0Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only