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Plasmid#171574PurposeExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membraneDepositorInsertBiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
UseTagsBiP signal peptide and mCherry-KDELExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-M plasmid with onboard mCherry cassette
Plasmid#169734PurposeEqualizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserttetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid with onboard mCherry cassette
Plasmid#169735PurposeEqualizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK R106A p2A mCherry
Plasmid#117688PurposeCMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencingDepositorInsertBARKrgs R106A peptide
UseTagsHA / FLAGExpressionMammalianMutationR106APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK D110A p2A mCherry
Plasmid#117690PurposeCMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencingDepositorInsertBARKrgs D110A peptide
UseTagsHA / FLAGExpressionMammalianMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E06: attB2r-mCherry-attB3
Plasmid#162903PurposeGateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteinsDepositorInsertmonomeric Cherry fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Cry2-mCherry- YAP-N-IDR
Plasmid#166446PurposeExpresses fusion of CRY2PHR with mCherry and YAP-N-IDRDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W
Plasmid#163172PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tagDepositorInsertYAP1 (YAP1 Human)
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry
Plasmid#159631Purposeexpresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoterDepositorInsertAst (AstA Fly)
UseLentiviralTagsHA, IRES, mCherry, and neurophysin-1ExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCaggs-NLS-PAmCherry1-GSS-EGFP
Plasmid#158000PurposeExpression of nucleus localized PAmCherry1 and EGFP fusion proteinDepositorInsertNLS-PAmCherry1-GSS-EGFP
UseTagsExpressionMammalianMutationPromoterActin promoter with CMV enhancerAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mCherry-3XNLS-WPRE
Plasmid#159951Purposenucleus markerDepositorInsertmCherry-3XNLS
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1B-mCherry-StreptagII
Plasmid#158744PurposeExpresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-B (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-290 and N-linked glycosylation sites r…PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXA13(+7A)-IDR-mCherry
Plasmid#145285PurposeBacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion ProteinDepositorInsertHOXA13(+7A)-IDR (HOXA13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXA13(WT)-IDR-mCherry
Plasmid#145284PurposeBacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion ProteinDepositorInsertHOXA13(WT)-IDR (HOXA13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+7A)-IDR-mCherry
Plasmid#145281PurposeBacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion ProteinDepositorInsertHOXD13(+7A)-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-026 mCherry(I-J)//Esp3I
Plasmid#130228PurposeEMMA Kit # 1000000119 extensionDepositorInsertmCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-027 mCherry(S-T)//Esp3I
Plasmid#130229PurposeEMMA Kit # 1000000119 extensionDepositorInsertmCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsH1R DRY->AAY-p2A-mCherry
Plasmid#137787PurposeVisualization of the Histamine-1 receptor DRY mutantDepositorInsertHuman histamine receptor; DRY mutant
UseTagsExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RnAT1aR DRY->AAY-p2A-mCherry
Plasmid#137784PurposeVisualization of the At1aR DRY mutantDepositorInsertRat At1a receptor; DRY mutant (Agtr1a Rat)
UseTagsExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only