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pLenti-hSyn-PROGERIN

Plasmid

#140189

Purpose

Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

Depositor

Lorenz Studer

Article

Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

Insert

Progerin (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Lamin A wildtype

Plasmid

#118709

Purpose

Lentiviral TET-ON inducible GFP-Lamin A wildtype

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Lamin A human wildtype (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV TRE3G (TET-ON)

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-HAPLN1

Plasmid

#200777

Purpose

Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

Depositor

Alexander Dityatev

Article

Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

Insert

Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)

Use

AAV

Tags

cfGFP

Expression

Mutation

Promoter

Synapsin

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LTA-Fc(DAPA)-AviTag-6xHis

Plasmid

#156568

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LTA (LTA Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-∆Hapln1

Plasmid

#200778

Purpose

Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

Depositor

Alexander Dityatev

Article

Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

Insert

Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)

Use

AAV

Tags

cfGFP

Expression

Mutation

deleted lectican binding domain (aminoacids 40-15…

Promoter

Synapsin

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

Plasmid

#172627

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR2

Plasmid

#116741

Purpose

Lentiviral expression of FGFR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR2 (FGFR2 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) PHLDB1

Plasmid

#107503

Purpose

Expresses PHLDB1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

PHLDB1 (PHLDB1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Nucleotide changes 1236 T>C, 1365 C>T, 1371…

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-Caveolin1-CMV-GFP

Plasmid

#83272

Purpose

Adenoviral expression of Caveolin1 with co-expression of GFP

Depositor

Andrew Brooks

Article

Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi:

Inserts

CAV1 (CAV1 Human)
eGFP

Use

Adenoviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-2 pMT2

Plasmid

#58732

Purpose

expression of mouse Alpha2 delta-2 in mammalian cells

Depositor

Annette Dolphin

Article

Barclay et al J Neurosci. 2001 Aug 15;21(16):6095-104.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

Plasmid

#171630

Purpose

Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

Depositor

Christopher A French, Chris French

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

Insert

BRD4-NUT (BRD4, NUTM1 Human)

Use

Tags

BioTAP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PCDH-METTL14-R298P

Plasmid

#141113

Purpose

Overexpression of METTL14 mutant in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

HMETTL14 (METTL14 Human)

Use

Tags

Expression

Mammalian

Mutation

Substitution - Missense, position 298, R➞P

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-JAG1-E228K

Plasmid

#116420

Purpose

Lentiviral expression of JAG1 E228K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

JAG1 (JAG1 Human)

Use

Lentiviral

Tags

Expression

Mutation

E228K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Progerin C661S

Plasmid

#118711

Purpose

Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Progerin C661S (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Mutation

C661S farnesylation mutant

Promoter

CMV TRE3G (TET-ON)

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT1_WT

Plasmid

#82234

Purpose

Gateway Donor vector containing STAT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT1 (STAT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HDAC1_WT

Plasmid

#82212

Purpose

Gateway Donor vector containing HDAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HDAC1 (HDAC1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-Flag-Mbd3

Plasmid

#52371

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Ub

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HAPLN1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156777

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HAPLN1 (HAPLN1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-Flag-Mbd3 delta1-70

Plasmid

#52372

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

deleted amino acids 1-70

Promoter

Ubiquitin

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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