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Plasmid#155265PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationLysine 51 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3-R70A
Plasmid#155264PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationArginine 70 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD111
Plasmid#78166PurposeExpresses Cas9 with the pLX311 backboneDepositorInsertCas9
UseLentiviralTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TGt
Plasmid#44508DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-T1GZmbh
Plasmid#44522DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-T1TCbt
Plasmid#44514DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TCbt
Plasmid#44515DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-UtrCH
Plasmid#124656PurposeF-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).DepositorInsertUtrophin (UTRN Human)
UseTagsEBFP2ExpressionMammalianMutationsee depositor comments belowPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
RCAS-sgATG7
Plasmid#75228PurposeAn adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor GammohDepositorInsertAutophagy Related 7 (Atg7 Mouse)
UseRetroviralTagsExpressionMutationPromoterU6Available sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1GZmbh
Plasmid#44516DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
sgGFP_3
Plasmid#78165PurposesgGFPDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g3 (BB24)
Plasmid#139459PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX311 DEDD
Plasmid#117745PurposeOpen reading frame vector encoding DEDDDepositorInsertDEDD1 (DEDD Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRISPRoff-v2.1
Plasmid#167981PurposeExpresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoterDepositorInsertCRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
UseTags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TETv4
Plasmid#167983PurposeExpresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoterDepositorInsertTETv4 (TET1CD-XTEN80-dCas9)
UseCRISPR and Synthetic BiologyTags3xNLS, TET1 catalytic domain, and tagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-B
Plasmid#104448PurposeExpression of human VAP-B fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
UseTagsEGFP and HAExpressionMammalianMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro
Plasmid#83306PurposeLentivirus vector to express guideRNA and dCas9 with puro resistant geneDepositorInsertsgRNA and dCas9 from pX330
UseLentiviralTagsFLAG and PA tagsExpressionMammalianMutationPromoterU6 for sgRNA and CBh for dCas9Available sinceDec. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000338462
Plasmid#78158PurposeshXPO1 targeting CDSDepositorInsertXPO1 (XPO1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000338401
Plasmid#78157PurposeshXPO1 targeting CDSDepositorInsertXPO1 (XPO1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
anxA2-GFP
Plasmid#107196PurposeExpresses human annexin A2-GFP fusion protein for live cell imagingDepositorInsertannexin A2 (ANXA2 Human)
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationAla residue 65 in human sequence replaced by Glu …PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
Plasmid#55613PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cellsDepositorInsert3xFLAG tagged HERC2 (WT) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationSilent mutations have been incorporated into the …PromoterAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000338399
Plasmid#78156PurposeshXPO1 targeting CDSDepositorInsertXPO1 (XPO1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-APP751
Plasmid#193772PurposeEntry vector for human APP isoform having 751 amino acid residuesDepositorInsertAPP (APP Human)
UseTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa-KIF5C 559-tdTomato-FKBP
Plasmid#64211PurposeKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tagsDepositorInsertKif5c (Kif5c Rat)
UseTagsFKBP and tdTomatoExpressionMammalianMutationmotor domain aa1-559Promoterchicken Beta ActinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APPSw;T719N
Plasmid#177094PurposeFor lentiviral transduction of APPsw; T719NDepositorInsertAPPsw;T719N (APP Human)
UseLentiviralTagsExpressionMutationT719N, K651N and M652L (please see depositor comm…PromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa-tdTomato-flag-BicD2 594-FKBP
Plasmid#64205PurposeBicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBPDepositorInsertBicaudal D protein (Bicd2 Mouse)
UseTagsFKBP, flag, and tdTomatoExpressionMammalianMutationno start methionine, aa1-594Promoterchicken Beta ActinAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-mRuby2
Plasmid#124657PurposePI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2DepositorInsertBTK (BTK Human)
UseTagsmRuby2ExpressionMammalianMutationcontains only the PH domain of Bruton tyrosine ki…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)
Plasmid#139447PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-GFP
Plasmid#130977PurposeExpression of KIF1C-GFP in mammalian cells.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTagsGFPExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro CXCR4 siRNA-1
Plasmid#12271DepositorInsertCXCR4 siRNA (CXCR4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)
Plasmid#139443PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro CXCR4 siRNA-2
Plasmid#12272DepositorInsertCXCR4 siRNA (CXCR4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC1-V16-eGFP
Plasmid#122033PurposeAAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expressionDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-GsCT
Plasmid#205517PurposeNuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).DepositorInsertnuc-GsCT (GNAS Synthetic, Human)
UseTagsHistone H2A and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g1 (BB34)
Plasmid#139452PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX311 HRAS
Plasmid#117749PurposeOpen reading frame vector encoding HRASDepositorInsertRASH1 (HRAS Human)
UseLentiviralTagsV5ExpressionMammalianMutationClosed vector so will not read through the V5 reg…PromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse DsRed-Monomer CASP9 C325A Mutant
Plasmid#198401PurposeExpresses Mouse DsRed-Monomer CASP9 C325A Mutant Fusion Protein in Mammalian CellsDepositorInsertMouse Caspase-9 C325A Mutant (Casp9 Mouse)
UseTagsDsRed-monomerExpressionMammalianMutationChanged Cysteine 325 to Alanine to eliminate prot…PromoterCMVAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX307 HRAS
Plasmid#117748PurposeOpen reading frame vector encoding HRASDepositorInsertRASH1 (HRAS Human)
UseLentiviralTagsV5ExpressionMammalianMutationClosed vector so will not read through the V5 reg…PromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
optoFcR
Plasmid#224291PurposeExpression of an optogenetic Fc ReceptorDepositorInsertFc receptor gamma (Fcer1g Mouse)
UseLentiviralTagsmScarlet-Cry2-olig and myristoylation sequenceExpressionMutationaa 45-86 onlyPromoterSFFVAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-Tet-O-Sox5-Puro
Plasmid#221667PurposeExpresses Sox5 in mammalian cellsDepositorInsertSox5 (Sox5 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only