pLX311 HRAS
(Plasmid
#117749)
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PurposeOpen reading frame vector encoding HRAS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX311
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Backbone manufacturerBroad Institute
- Total vector size (bp) 8797
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRASH1
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SpeciesH. sapiens (human)
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Insert Size (bp)509
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MutationClosed vector so will not read through the V5 region
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Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
- Promoter EF1a
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX311 HRAS was a gift from William Hahn (Addgene plasmid # 117749 ; http://n2t.net/addgene:117749 ; RRID:Addgene_117749) -
For your References section:
MCL1 and DEDD promote urothelial carcinoma progression. Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai A, Rosenberg JE, Hahn WC. Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 10.1158/1541-7786.MCR-18-0963. 10.1158/1541-7786.MCR-18-0963 PubMed 30777879