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pCAG-NLS-HA-Dre
Plasmid#51272PurposeComponent of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)DepositorInsertNLS-HA-Dre
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET24b-SUMO-BG2.4
Plasmid#199438Purposeexpresses BAmGreen2.4 in bacterial cells with a SUMO tagDepositorInsertBAMGreen2.4
UseTagshis tag and sumoExpressionBacterialMutationPromoterT7Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN-TetR-mCherry
Plasmid#59417Purposeretroviral construct expressing the Tet repressor (TetR) tagged with mCherryDepositorInsertTet repressor protein (TetR)
UseRetroviralTagsmCherryExpressionMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-COL1A1
Plasmid#66602PurposeExpresses procollagen 1 alpha 1 as a GFP fusionDepositorInsertCOL1A1 (COL1A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MetGFP
Plasmid#37560DepositorInsertMET (MET Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Rheb-N153T
Plasmid#19997DepositorInsertRas homolog enriched in brain 1 - N153T (RHEB Human)
UseTagsFLAGExpressionMammalianMutationChanged Asparagine 153 to ThreoninePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCh-Climp63
Plasmid#136293Purposeexpression of Climp63DepositorInsertCytoskeletal-linking protein 63 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-eGFP
Plasmid#116926PurposeRetrovirus expressing eGFP with BBSI cloning sites for sgRNADepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA
Plasmid#160141PurposeCre- dependent expression of Sun1GFP for nuclei isolationDepositorInsertSun1-GFP (Sun1 Mouse)
UseAAVTagsGFPExpressionMammalianMutationN-truncated: removed amino acids 1-207, retained …PromoterEf1aAvailable sinceMarch 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MyrPalm-CKAR
Plasmid#14862DepositorInsertC kinase activity reporter
UseTagsCFP, Myr/Palm, and YFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAPM-miR30-IFIH1-ts3
Plasmid#174261PurposeIFIH1 knockdownDepositorInsertIFIH1 shRNA (IFIH1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-KRAB-blast
Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABExpressionMutationD10A and H840APromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PEmax-IRES-ZeoR
Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)
Plasmid#129405Purposecircular Pepper RNADepositorInsertcircular F30-Pepper(TAR Variant-2)
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
EMTB-3XGFP
Plasmid#26741DepositorInsertensconsin (MAP7 Human)
UseProbe for microtubulesTags3x EGFP and MycExpressionMammalianMutationOnly contains the microtubule binding-domain of e…PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
MI-Luciferase-IRES-mCherry
Plasmid#75020PurposeEncodes the expression of Luciferase and mCherryDepositorInsertsfirefly Luciferase
mCherry
UseLuciferase and RetroviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/TO-eGFP-REV7
Plasmid#114128PurposeExpresses N-terminally tagged eGFP-REV7 in mammalian cellsDepositorInsertREV7 (MAD2L2 Human)
UseGenomic integration, tetracycline inducibleTagseGFPExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-hsRPA70 (MSW#473)
Plasmid#208067PurposeExpression of GFP-tagged hsRPA1 in human cellsDepositorInsertRPA1 (RPA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
YAP1 (S6A) - V5 in pLX304
Plasmid#42562DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationS61A, S109A, S127A, S128A, S131A, S163A, S164A, S…PromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM-W
Plasmid#211701PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.eGFP.2A.P301L-Tau
Plasmid#140425PurposeAAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTauDepositorInserteGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
UseAAVTagseGFPExpressionMutationchanged Proline 301 to LeucinePromoterCBAAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP/GCP60
Plasmid#180633PurposeExpression of GCP60DepositorInsertGCP60 (ACBD3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS-HA
Plasmid#130910PurposeExpresses human cGAS-HA; Puromycin selection markerDepositorInsertcGAS
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP955
Plasmid#98784PurposeAccessory Plasmid for PACEDepositorInsertproB-lysozyme-ggs-ENLYFQS-ggs-T7RNAP//T7pro-gIII-lux
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-C1
Plasmid#54495PurposeLocalization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517DepositorTypeEmpty backboneUseTagsPA-mCherry1ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9
Plasmid#135964PurposeExpresses SauriCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
Plasmid#129631PurposeVisualization of Akt and ERK activityDepositorInsertH2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 NLS1, 5F-L YFP
Plasmid#84913PurposeMammalian expresion of TDP-43 NLS1, 5F-L YFPDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationK82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…PromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PsppA-mCherry
Plasmid#225428PurposePlasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherryDepositorInsertmCherry, Cas9, SgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPsppA upstream of mCherry and Cas9, P3 upstream o…Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
m6A-Tracer-NES
Plasmid#159607PurposeConstitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.DepositorInsertm6A-Tracer-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-TurboID-NES_pLX304
Plasmid#202019Purposeexpresses GFP-TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-GFP-TurboID-NES
UseLentiviralTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-53BP1
Plasmid#60813Purposemammalian expression vectorDepositorInsert53BP1 (TP53BP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFB1_hPMS2
Plasmid#129425PurposeExpression human PMS2 protein using baculovirusDepositorInserthuman PMS2 (PMS2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC840 - pAAV 5xATF6 secNLuc
Plasmid#82497PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivoDepositorInsertsecNLuc
UseAAVTagsExpressionMammalianMutationPromoter5x(ATF6 binding site) c-Fos minimal promoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EOS-C(3+)-EiP
Plasmid#21313PurposeLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterEOS-C(3+) promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
CLYBL_Bxb1-GA_LP_Dual_TC
Plasmid#229776PurposeCLYBL targeting construct with the Bxb1-GA landing pad cassette.DepositorInsertFRT-mScarlet-attP(GA)-PuroR-F3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpGKAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpRY-P2A-GFP
Plasmid#159981PurposePrime editing in mammalian cellsDepositorInsertPE2-SpRY-P2A-GFP
UseTagsBpSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only
