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Plasmid#16756DepositorInsertFLAG-82-716-Dsh3 (DVL3 Human)
UseTagsflagExpressionMammalianMutationaa 82-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras 12V (35S)
Plasmid#12588DepositorInsertHRas 12V 35S (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation12V, 35SPromoterAvailable sinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEN_TGmiR_Gb2-K
Plasmid#25766PurposeEntry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.DepositorInsertGb2 miR-shRNA (Gnb2 Mouse)
UseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6I4Q5
Plasmid#103101PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaCoiledCoil-LIN-41
Plasmid#52720Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 328–447PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4MS2-4H
Plasmid#108370PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 MUT
Plasmid#110124PurposeExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activityDepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutation4 mutations (KSLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
XE254 pCDNA3.1(zeo)-hDsh3-497-716
Plasmid#16755DepositorInsertFLAG-497-716-Dsh3 (DVL3 Human)
UseTagsflagExpressionMammalianMutationaa 497-716PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR173
Plasmid#106058PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)DepositorInsertOpto-GPR173 (GPR173 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR31
Plasmid#106014PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)DepositorInsertOpto-GPR31 (GPR31 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus-TmiR-G13-G12
Plasmid#25742PurposeLentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.DepositorInsertG alpha 13 and 12 miR-shRNA
UseLentiviral and RNAiTagsVenusExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4C (216-233) (pGEX2T(TEV))
Plasmid#80635PurposeResidues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202DepositorInsertCHMP4C (CHMP4C Human)
UseTagsGSTExpressionBacterialMutationResidues 216-233PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F3UXG6
Plasmid#103108PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
RF-118-UB-BRCA1
Plasmid#78960PurposeExpression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertBRCA1 (BRCA1 Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-1155PromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-C7TEQ6
Plasmid#103092PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertC7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR25
Plasmid#106011PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR25; Opto-GPR25; B1)DepositorInsertOpto-GPR25 (GPR25 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER
Plasmid#16753DepositorInsertFLAG-hDsh3-deltaCTER (DVL3 Human)
UseTagsflagExpressionMammalianMutationdelta CTERPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR-mDIXDC1 WT
Plasmid#61216PurposeEntry clone encoding long isoform of mouse DIXDC1DepositorInsertDIXDC1 (Dixdc1 Mouse)
UseDonr clone (gateway)TagsExpressionMutationPromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-4MS2-xrRNA-4H
Plasmid#108371PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -