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Plasmid#167837PurposeEncodes OptoLAC1B circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (LAA)ExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL659
Plasmid#167838PurposeEncodes OptoLAC2B circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38
Plasmid#65516PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edCit-attR1 and attR2-edCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCLX-pTF-R1-DEST-R2-EBR65
Plasmid#45952DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGWB501NL3F10H
Plasmid#141286PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRETgc-2in1-NN
Plasmid#105124PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmEGFP/mCherryExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP34
Plasmid#65512PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPF144
Plasmid#106372PurposeC. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusADepositorInsertPcwp2-gusA (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPcwp2Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP37
Plasmid#65515PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-Cer-cMycExpressionYeastMutationPromoterpPMA1Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP36
Plasmid#65514PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbA8c-RFP
Plasmid#35272DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
PibpA GFP pZa
Plasmid#170076PurposeGFP expression under the control of E. coli ibpA promoterDepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPibpAAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PfxsA GFP pZa
Plasmid#170075PurposeGFP expression under the control of E. coli fxsA promoterDepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPfxsAAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP43
Plasmid#65519PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP39
Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-Renilla-ccdB
Plasmid#87076PurposeLentiviral Gateway-compatible expression vector backbone with an N-terminal Renilla luciferaseDepositorTypeEmpty backboneUseLentiviralTagsRenilla luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS6c-RFP
Plasmid#35293DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLlacO-1Available sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only