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MSCV-PIG-N3FLAG-TAF10

Plasmid

#105990

Purpose

retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

FLAG tagged TAF10 (Taf10 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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Plxna3-Fc-His

Plasmid

#72124

Purpose

Expresses the extracellular region of the PlexinA3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna3 (Plxna3 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13A tail 361-1749

Plasmid

#134622

Purpose

Mammalian expression of mouse KIF13A 361-1749

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13A (Kif13a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 360 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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3410 pBM272 Bcl-XL

Plasmid

#8772

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bcl-XL (Bcl2l1 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
YFP-mDia1FH2deltaN

Plasmid

#25419

Depositor

Arthur Alberts

Article

Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

Insert

diaph1 (Diaph1 Mouse)

Use

Tags

YFP A206K

Expression

Mammalian

Mutation

mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

Promoter

Available since

July 20, 2010

Availability

Academic Institutions and Nonprofits only
FLAG-Foxo1-ADA 6KR

Plasmid

#17561

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1-ADA 6KR (Foxo1 Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
CRT-GFP pcDNA3.1

Plasmid

#80978

Purpose

mammalian expression of CRT-GFP

Depositor

Peter Steyger

Article

Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

Insert

CRT (Calr Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

no KDEL

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
Myc-Axin2

Plasmid

#21279

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 (Axin2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
Lenti-sgHcn1#2/Cre

Plasmid

#193218

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Hcn1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgHcn1#2 (Hcn1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMK277 (mClover-NeoR)

Plasmid

#72793

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Neo

Use

CRISPR

Tags

mClover

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

Plasmid

#127864

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(R306C)

Plasmid

#110188

Purpose

Expression of mouse MeCP2 (R306C) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Arg 306 Cys

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd9

Plasmid

#42261

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 9 (Fzd9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pJFRC4-3XUAS-IVS-mCD8::GFP

Plasmid

#26217

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

mCD8::GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-TGFB2

Plasmid

#164428

Purpose

AAV plasmid expressing TGFB2 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb2 (Tgfb2 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human red opsin

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-PGK-Chst3

Plasmid

#193024

Purpose

AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

Depositor

Bart Nieuwenhuis

Article

Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

Insert

Chst3 (Chst3 Mouse)

Use

AAV

Tags

3xFLAG and myc-tag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Ribo-jGCaMP8f

Plasmid

#167573

Purpose

Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Ribo-GCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-5aHA

Plasmid

#18032

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5a (Wnt5a Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
shMEK1/2 v2 neo

Plasmid

#72570

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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