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Plasmid#146962PurposeBacterial Expression of NcQde-2_506-938-D603KDepositorInsertNcQde-2_506-938-D603K
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2_506-938-K638E_M
Plasmid#146963PurposeBacterial Expression of NcQde-2_506-938-K638EDepositorInsertNcQde-2_506-938-K638E
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41P-HsNot2_344-540_M
Plasmid#146946PurposeBacterial Expression of HsNot2_344-540DepositorInsertHsNot2_344-540 (CNOT2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmGW182-RBD_F
Plasmid#146290PurposeBacterial Expression of DmGW182-RBDDepositorInsertDmGW182-RBD (gw Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-3C-HsRCK_296-472_E
Plasmid#146209PurposeBacterial Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmTral_1-84_C
Plasmid#146082PurposeBacterial Expression of DmTral_1-84DepositorInsertDmTral_1-84 (tral Fly)
UseTagsExpressionBacterialMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmEDC3_1-101_D
Plasmid#146126PurposeBacterial Expression of DmEDC3_1-101DepositorInsertDmEDC3_1-101 (Edc3 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM41P-HsUPF3b-iso2_145-470_K
Plasmid#146754PurposeBacterial Expression of HsUPF3biso2_145-470DepositorInsertHsUPF3biso2_145-470 (UPF3B Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41P-HsNot1iso1_1597-2376_V
Plasmid#147746PurposeBacterial Expression of HsNot1iso1_1597-2376DepositorInsertHsNot1iso1_1597-2376 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NdeI-NcQde-2_V
Plasmid#147747PurposeBacterial Expression of NcQde-2DepositorInsertNcQde-2
UseTagsExpressionBacterialMutation1 non silent mutation P761S compared to the seque…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_CoA-Snifit(G41)
Plasmid#197939PurposeExpression of CoA-Snifit in bacterial cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionBacterialMutationPanK=PanK-D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_CoA-Snifit(G41S)
Plasmid#197940PurposeExpression of CoA-Snifit in bacterial cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionBacterialMutationPanK=PanK-G41S, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_CoA-Snifit(V97T)
Plasmid#197941PurposeExpression of CoA-Snifit in bacterial cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionBacterialMutationPanK=PanK-V97T, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K403A
Plasmid#195612PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationA1463G, A1464CPromoterT7Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K223I
Plasmid#195615PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activityDepositorInsertI-SceI
UseTagsExpressionBacterialMutationC2694G, G2345C, G2351C, A3365T, A3366TPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K122I
Plasmid#195614PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activityDepositorInsertI-SceI
UseTagsExpressionBacterialMutationC2458G, A2826T, A2827TPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC D218A
Plasmid#195608PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT928C, T929C, A942CPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K301A
Plasmid#195611PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationA822G, A823C, A824TPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2_506-938-H609A_O
Plasmid#147083PurposeBacterial Expression of NcQde-2_506-938-H609ADepositorInsertNcQde-2_506-938-H609A
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2_506-938-T642E_O
Plasmid#147084PurposeBacterial Expression of NcQde-2_506-938-T642EDepositorInsertNcQde-2_506-938-T642E
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only