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pET15b/EYFP-GgVcl 1-258

Plasmid

#46263

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl 1-258 (VCL Chicken)

Use

Tags

EYFP and His

Expression

Bacterial

Mutation

aa 1-258 (VD1)

Promoter

T7

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pet 3d double tentacle delete CP

Plasmid

#13301

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)

Use

Tags

Expression

Bacterial

Mutation

Capping protein alpha 1 (with 28 amino acids dele…

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pET-21a(+) SurE D9A

Plasmid

#12686

Depositor

SeWon Suh

Article

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE

Use

Tags

His

Expression

Bacterial

Mutation

changed Aspartate 9 to Alanine

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pet28a-ClbB-NRPS-NHis

Plasmid

#49217

Purpose

NRPS module of ClbB containing CAT domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbB-NRPS

Use

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa4-1080 of reference sequence NP_754362…

Promoter

T7

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) no-tags pET

Plasmid

#8493

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HoxA9 (Hoxa9 Mouse)

Use

Tags

Expression

Bacterial

Mutation

makes protein by Western

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET22b-EC12-129/138

Plasmid

#47926

Purpose

Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Use

Tags

6xHis and TEV Cleavage

Expression

Bacterial

Mutation

Mutations C9A, K129C, D138C

Promoter

T7

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pET-28b(+)-DR RecR(34-220)

Plasmid

#12618

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR(34-220)

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-33

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pet 3d alpha tentacle delete CP

Plasmid

#13299

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits (CAPZB Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pet 3d beta tentacle delete CP

Plasmid

#13300

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 1 (deletion 28 amino acids ) (CAPZB Chicken)

Use

Tags

Expression

Bacterial

Mutation

CP alpha subunit is intact with a mutated beta C-…

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
Rv2002 M3/pET-28b(+)N-tag

Plasmid

#13057

Depositor

SeWon Suh

Article

Yang et al Proc Natl Acad Sci U S A. 2003 Jan 21. 1

Insert

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Use

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Use

Tags

6xHis and mScarlet-I

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-SAV

Plasmid

#191197

Purpose

Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Streptavidin fusion

Use

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Use

Tags

6xHis and mScarlet-I

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-SAV

Plasmid

#191200

Purpose

Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & Streptavidin fusion

Use

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-MfnG-TEV-His

Plasmid

#191114

Purpose

Expresses MfnG-TEV-His in E. coli

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Article

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Use

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pETDuet-AtRBX1-T7-AtASK1-T7-AtCUL1-S

Plasmid

#222715

Purpose

Expression AtRBX1-T7, AtASK1-T7 and AtCUL1-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtRBX1 (RBX1 Mustard Weed)
AtASK1 (SKP1 Mustard Weed)
AtCUL1 (CUL1 Mustard Weed)

Use

Tags

S-Tag and T7

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

Plasmid

#187708

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-380

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT (492)

Plasmid

#187709

Purpose

Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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