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Addgene

Rv2002 M3/pET-28b(+)N-tag
(Plasmid #13057)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13057 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-28b(+)
  • Backbone manufacturer
    Merck
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
  • Alt name
    Rv2002 M3
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    783
  • GenBank ID
    BX842578
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rv2002 M3/pET-28b(+)N-tag was a gift from SeWon Suh (Addgene plasmid # 13057 ; http://n2t.net/addgene:13057 ; RRID:Addgene_13057)
  • For your References section:

    Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis. Yang JK, Park MS, Waldo GS, Suh SW. Proc Natl Acad Sci U S A. 2003 Jan 21. 100(2):455-60. 10.1073/pnas.0137017100 PubMed 12524453