Rv2002 M3/pET-28b(+)N-tag
(Plasmid
#13057)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28b(+)
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Backbone manufacturerMerck
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
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Alt nameRv2002 M3
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SpeciesMycobacterium tuberculosis
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Insert Size (bp)783
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GenBank IDBX842578
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rv2002 M3/pET-28b(+)N-tag was a gift from SeWon Suh (Addgene plasmid # 13057 ; http://n2t.net/addgene:13057 ; RRID:Addgene_13057) -
For your References section:
Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis. Yang JK, Park MS, Waldo GS, Suh SW. Proc Natl Acad Sci U S A. 2003 Jan 21. 100(2):455-60. 10.1073/pnas.0137017100 PubMed 12524453