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We narrowed to 3,399 results for: biorxiv

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Showing: 3021 - 3040 of 3399 results

  1. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Promoter
    4xHRE_YB TATA and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  9. pSBL_DK202

    Plasmid
    #156426
    Purpose
    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    EGFP-His
    Expression
    Mammalian
    Mutation
    aa 42-275
    Promoter
    CMV
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156426-plasmid-map-sequence-id-306303

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  12. pAAV-CMV-dSa VP64 Neurog2

    Plasmid
    #99695
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    Sept. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99695-plasmid-map-sequence-id-194570

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  13. pAAV-CMV-dSa VP64 Actc1

    Plasmid
    #99691
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    99691-plasmid-map-sequence-id-194530

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  14. pET28a-6xHis-SSF1

    Plasmid
    #255298
    Purpose
    SSF1 (1-473) with an N-terminal 6X poly-histidine tag and a TEV recognition site sub-cloned into pET28 plasmids with Kanamycin bacterial resistance, were used for protein expression
    Depositor
    Richard Kriwacki
    Article
    Dogra et al bioRxiv 2025.03.01.640913
    Insert
    SSF1 (PPAN Human)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255298-plasmid-map-sequence-id-509456

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  15. pET28a-6xHis-SURF6-C19S

    Plasmid
    #255297
    Purpose
    Single cysteine mutant (C19S) of SURF6 (1-361) with an N-terminal 6X poly-histidine tag and a TEV recognition site sub-cloned into pET28 plasmids with Kanamycin bacterial resistance.
    Depositor
    Richard Kriwacki
    Article
    Dogra et al bioRxiv 2025.03.01.640913
    Insert
    SURF6-C19S (SURF6 Human)
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Mutated cysteine 19 to serine
    Promoter
    T7
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255297-plasmid-map-sequence-id-509450

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  16. pET28a-6xHis-SURF6

    Plasmid
    #255296
    Purpose
    SURF6 (1-361) with an N-terminal 6X poly-histidine tag and a TEV recognition site sub-cloned into pET28 plasmids with Kanamycin bacterial resistance, were used for protein expression.
    Depositor
    Richard Kriwacki
    Article
    Dogra et al bioRxiv 2025.03.01.640913
    Insert
    SURF6 (SURF6 Human)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    255296-plasmid-map-sequence-id-509016

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  17. Epac-S-H334

    Plasmid
    #245738
    Purpose
    FLIM biosensor for real-time cAMP monitoring, no nuclear membrane affinity, with nuclear photoactivatable mCherry. mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST)-2A-PAmCherry-3xNLS
    Depositor
    Kees Jalink
    Article
    Mukherjee et al bioRxiv 2025.07.11.664338
    Insert
    mTurq2del_EPACdDEPCD_Q270E_L777A_K778A_tdblcp173V(st)
    Tags
    T2A, Photoactivatable mCherry-3xNLS
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    EF-1a
    Available Since
    April 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    245738-plasmid-map-sequence-id-488744

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  18. Epac-S-H250

    Plasmid
    #245739
    Purpose
    FLIM biosensor for real-time cAMP monitoring, with nuclear photoactivatable mCherry to label nuclei of selected cells. mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Venus(ST)-2A-PAmCherry-HNS
    Depositor
    Kees Jalink
    Article
    Mukherjee et al bioRxiv 2025.07.11.664338
    Insert
    mTurq2del_EPACdDEPCD_Q270E_tdblcp173V(st)
    Tags
    T2A, Photoactivatable mCherry-HNS
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Available Since
    April 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    245739-plasmid-map-sequence-id-488737

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Showing: 3021 - 3040 of 3399 results