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pTE_20 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-BARD1-NLS-HbaUTR

Plasmid

#252016

Purpose

In vitro transcription of Cas9-BARD1 TruEditor for mRNA generation

Depositor

Neville Sanjana

Insert

Cas9-BARD1

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_19 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-NLS-HbaUTR

Plasmid

#252015

Purpose

In vitro transcription of Cas9 only for mRNA generation

Depositor

Neville Sanjana

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_22 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-SLX1A-NLS-HbaUTR

Plasmid

#252018

Purpose

In vitro transcription of Cas9-SLX1A TruEditor for mRNA generation

Depositor

Neville Sanjana

Insert

Cas9-SLX1A

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_18 - pCMV-Cas9-BLM(1-193)

Plasmid

#252014

Purpose

Mammalian expression of Cas9-BLM(2-194) TruEditor

Depositor

Neville Sanjana

Insert

Cas9-BLM(2-194)

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_17 - pLenti_EFS-Cas9-BARD1(32-117)-2A-BlastR

Plasmid

#252013

Purpose

Lentiviral mammalian expression of Cas9-BARD1(32-117) TruEditor

Depositor

Neville Sanjana

Insert

Cas9-BARD1(32-117)

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_16 - pLenti_EFS-Cas9-BLM(1-193)-2A-BlastR

Plasmid

#252012

Purpose

Lentiviral mammalian expression of Cas9-BLM(2-194) TruEditor

Depositor

Neville Sanjana

Insert

Cas9-BLM(2-194)

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_07 - pLenti_EFS-Cas9-XTEN-FEN1-2A-BlastR

Plasmid

#252003

Purpose

Lentiviral mammalian expression of Cas9-FEN1 TruEditor

Depositor

Neville Sanjana

Insert

Cas9-FEN1

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_06 - pLenti_EFS-Cas9-XTEN-RAD18-2A-BlastR

Plasmid

#252002

Purpose

Lentiviral mammalian expression of Cas9-RAD18 TruEditor

Depositor

Neville Sanjana

Insert

Cas9-RAD18

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_05 - pLenti_EFS-Cas9-XTEN-SLX1A-2A-BlastR

Plasmid

#252001

Purpose

Lentiviral mammalian expression of Cas9-SLX1A TruEditor

Depositor

Neville Sanjana

Insert

Cas9-SLX1A

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_04 - pLenti_EFS-Cas9-XTEN-ZNF146-2A-BlastR

Plasmid

#252000

Purpose

Lentiviral mammalian expression of Cas9-ZNF146 TruEditor

Depositor

Neville Sanjana

Insert

Cas9-ZNF146

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_03 - pLenti_EFS-Cas9-XTEN-BARD1-2A-BlastR

Plasmid

#251999

Purpose

Lentiviral mammalian expression of Cas9-BARD1 TruEditor

Depositor

Neville Sanjana

Insert

Cas9-BARD1

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_01 - pLenti_EFS-Cas9-XTEN-ZRANB3-2A-BlastR

Plasmid

#251997

Purpose

Lentiviral mammalian expression of Cas9-ZRANB3 TruEditor

Depositor

Neville Sanjana

Insert

Cas9-ZRANB3

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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YY229: pAAV_TRE::GFP- GEMINI(A)-P1N4_hSyn::rtTA3G

Plasmid

#228889

Purpose

AAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3G under the control of a hSyn promotor

Depositor

Dingchang Lin

Article

Yan et al bioRxiv [Preprint]. 2025 Jul 18:2025.07.

Insert

GFP-GEMINI(A); rtTA3G

Use

AAV

Tags

gfp

Promoter

TRE; hsyn

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A)

Plasmid

#228881

Purpose

PiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor.

Depositor

Dingchang Lin

Article

Yan et al bioRxiv [Preprint]. 2025 Jul 18:2025.07.

Insert

Building blocks of GEMINI for intracellular assembly

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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YY105: pPB_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

Plasmid

#228882

Purpose

PiggyBac plasmid expressing the HaloTag-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3 and PuroR under the control of a UbC promoter.

Depositor

Dingchang Lin

Article

Yan et al bioRxiv [Preprint]. 2025 Jul 18:2025.07.

Insert

HaloTag-fused GEMINI(A)

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pPig-hEF1a-Cry2PHR-LRP6c-Puro-Blind

Plasmid

#249712

Purpose

Expresses optogenetic LRP6 in mammalian cells; no fluorescent proteins. Plasmid contains a puromycin selection marker.

Depositor

Max Wilson

Article

Rosen et al bioRxiv [Preprint]. 2025 May 23:2025.02.

Inserts

Cry2-LRP6 (CRY2, LRP6 Mustard Weed, Human)
P2A-PuroR

Expression

Mammalian

Promoter

hEF1a

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A)

Plasmid

#218776

Purpose

Expresses dCAS9-DNMT3A-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A-KRAB (dCas9-3A-KRAB)

Plasmid

#218781

Purpose

Expresses dCAS9-DNMT3A-KRAB fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A-3L)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-mutDNMT3A(R882H)(dCas9-mut3A)

Plasmid

#218788

Purpose

Expresses dCAS9- R882H Mutated DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-MutDNMT3A (dCas9-Mut3A (R882H))
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-RVdG-MCP:oScarlet:KASH-6xMBS

Plasmid

#231011

Purpose

CVS N2c RVdG genome plasmid. Utilizes MS2 tagging to deliver the MCP:oScarlet:KASH transcript to the outer nuclear membrane. Insert high complexity barcode between SacII RE sites distal to 6xMBS site.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH-6xMBS-SacIIMCS

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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