We narrowed to 4,011 results for: biorxiv
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Plasmid#229793PurposeBxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterTRE and CAGAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
p35S-FHA-SpAGO4a_gDNA
Plasmid#216841PurposeN-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a gDNA under regulation of CaMV 35S promoterDepositorInsertARGONAUTE4
TagsFlag-HAExpressionPlantPromoterCaMV 35SAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
p35S-FHA-SpAGO4a_cDNA
Plasmid#216842PurposeN-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a cDNA under regulation of CaMV 35S promoterDepositorInsertARGONAUTE4
TagsFlag-HAExpressionPlantPromoterCaMV 35SAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG
Plasmid#200792PurposeMammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
TagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutationΔ954-1027PromoterCMV & T7Available SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG
Plasmid#200798PurposeMammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
TagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutation411-680PromoterCMV & T7Available SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-pol2-Smarcd1-Myc
Plasmid#227716PurposeExpresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.DepositorAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3346 PB-nicking guide-blast
Plasmid#226665PurposePiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidinDepositorInsertprotective nicking sgRNA
UseCRISPRExpressionMammalianPromoterhU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-rTetR(SE)-KRAB-2A-mTurquoise
Plasmid#225690PurposeLentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLSDepositorInsertrTetR-ZNF10 KRAB
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1302-AAV-EFSNC-dCjCas9-KRAB
Plasmid#223147PurposeExpression of KRAB with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1312-AAV-EFSNC-dSaCas9-KRAB
Plasmid#223157PurposeExpression of KRAB with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG7 CTF-FLAG
Plasmid#217705PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGR7 (ADGRG7 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-415Available SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-3xFLAG-SMAD1-active
Plasmid#222579PurposePiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1DepositorAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
Plasmid#222990PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialPromotertacAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA
Plasmid#218870PurposeMammalian expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
ExpressionMammalianMutationS99A F102G R168PPromoterCAGAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2(no bind)-6xHis
Plasmid#218887PurposeBacterial expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
Tags6xHisExpressionBacterialMutationS99A F102G R168PPromoterT7Available SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
mFzd4CRDL(42-203)-6xHis_pAcGP67a
Plasmid#216379PurposeBaculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cellsDepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags6xHis and GP64 signal sequenceExpressionInsectPromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-IRES-puro6 gal4DBD-HIFCAD myc tag
Plasmid#207171Purposeexpression plasmid for Gal4DBD-HIF-CAD.myc expressionDepositorInsertGal4DBD-HIF1a-CAD Myc tag linker
Tags2xMyc tagExpressionMammalianPromoterEF1aAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet_OPTN-thrombin-GST
Plasmid#216843PurposeFor expression in E. coli of OPTN (Internal ref. SMC1789)DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-GST-TEV-SINTBAD-mCherry
Plasmid#208874PurposeFor expression in insect cells of SINTBAD-mCherryDepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only