We narrowed to 3,975 results for: biorxiv

1. Coexpressed mRuby3 VLP

   Plasmid

   #182431

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-GFP
   deltaVP1
   VP2C-mRuby3
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1, GAL10, and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Linked Free NES (M)

   Plasmid

   #182436

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Insert

   FPPS(M)-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Coexpressed Free NES (M)

   Plasmid

   #182437

   Purpose

   Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   FPPS(M)
   NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10 and GAL7

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFR-mTq2-antiFRET-P2A-CH-GECO2.1

   Plasmid

   #191470

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   CH-GECO2.1
   

   Tags

   His-mTurquoise2-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFR-mScarletI-antiFRET-P2A-jGCaMP7c

   Plasmid

   #191461

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   jGCaMP7c
   

   Tags

   His-mScarletI-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGlomyc-Ovol1

   Plasmid

   #172871

   Purpose

   Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

   Depositor

   Renee van Amerongen

   Article

   Mourao et al bioRxiv 2021.06.22.449461

   Insert

   Ovol1 (Ovol1 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDisplay-GRAB_g5-HT3.0

   Plasmid

   #208711

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-REPORT(EF1a)-TTN

   Plasmid

   #172327

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

   Plasmid

   #207664

   Purpose

   Neuropeptide degradation

   Depositor

   Sung Han

   Article

   Kim et al bioRxiv 2023.01.19.524797

   Insert

   NEP (MME Human)
   

   Use

   AAV and Cre/Lox

   Tags

   mRUBY3

   Mutation

   Addition of signaling peptide (POMC) on the N-ter…

   Promoter

   hSynapsin

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Mutation

    DR1 A>T

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid

    #218874

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    Synapsin

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATP5F1B-turboGFP

    Plasmid

    #239795

    Purpose

    Expresses human ATP5F1B tagged with turboGFP

    Depositor

    Samantha Lewis

    Article

    Rose et al bioRxiv [Preprint]. 2025 Mar 25:2025.03.

    Insert

    ATP5F1b (ATP5F1B Human)
    

    Tags

    turboGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

    Plasmid

    #217719

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2522

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC1_oROS-HT

    Plasmid

    #216413

    Purpose

    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

    Plasmid

    #217686

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2516

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB_g5-HT3.0mut

    Plasmid

    #208718

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

    Plasmid

    #230988

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Cre-dependent, membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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