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pTRE3G-puro_V5-LOV-Tb-myc-MIRO1-TM-KQR (OMM)

Plasmid

#253797

Purpose

Express V5-LOV-Turbo2-myc-Miro1-TM-KQR (OMM) under TRE3G (tetracycline inducible) promoter

Depositor

Xinnan Wang

Article

Kwak et al bioRxiv [Preprint]. 2025 Dec 23:2025.12.

Insert

V5-LOV-Tb-myc-Miro1-TM-KQR (OMM) (RHOT1 Human)

Use

Lentiviral; Tetracycline inducible promoter

Expression

Mammalian

Promoter

pTRE_tetracycline inducible

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00134_PGK-mNeonGreen-syn_pA-EF1alpha-TagBFP-syn_pA

Plasmid

#252538

Purpose

Constitutive promoters with downstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00135_EF1alpha-TagBFP-syn_pA-PGK-mNeonGreen-syn_pA

Plasmid

#252539

Purpose

Constitutive promoters with upstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01701_CMV-rtTA-betaglobin_pA-CMV-PuroR-bGH

Plasmid

#252540

Purpose

Secondary PiggyBac vector for puromycin selection and expression of transcriptional activator

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA

Expression

Mammalian

Promoter

CMV (human cytomegalovirus immediate early enhanc…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01416_TRE-TagBFP-bGH-EF1alpha-mRuby2-SV40

Plasmid

#252541

Purpose

Inducible gene and constitutive reporter with downstream tandem syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01417_TRE-TagBFP-bGH-(EF1alpha-mRuby2-SV40)

Plasmid

#252542

Purpose

Inducible gene and constitutive reporter with convergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01418_(TRE-TagBFP-bGH)-EF1alpha-mRuby2-SV40

Plasmid

#252543

Purpose

Inducible gene and constitutive reporter with divergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG04062_pLentiX1-EF1alpha-GapR-3xFLAG-NLS-EGFP-bGH

Plasmid

#252547

Purpose

Lentiviral expression of GapR-EGFP fusion protein for GapRUN assay

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

GapR-3xFLAG-NLS-EGFP

Use

Lentiviral

Tags

3xFLAG-SV40 NLS

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha)

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG02752_pLentiX1-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252548

Purpose

Lentiviral expression of the rtTA transcriptional activator with a fluorescent reporter

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ULK1-ADA

Plasmid

#249534

Purpose

Expressing human full length ULK1 with P433A/V434D/P435A mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

Flag-tag and strep-tag

Expression

Mammalian

Mutation

P433A, V434D and P435A

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-1-835-mcherry

Plasmid

#249729

Purpose

Expressing human ULK1-1-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-1-290-mcherrry

Plasmid

#249732

Purpose

Expressing human ULK1-1-290 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-290

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-290-835-mcherry

Plasmid

#249733

Purpose

Expressing human ULK1-290-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

290-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-ATG13-1-230

Plasmid

#250343

Purpose

Expressing human ATG13 (aa. 1-230)

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Tags

GFP

Expression

Mammalian

Mutation

only amino acids 1-230

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-ATG13-1-230(HF|DD)

Plasmid

#250344

Purpose

Expressing human ATG13 (1-230) with H214D/F215D mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Tags

GFP

Expression

Mammalian

Mutation

only amino acids 1-230 with H214D and F215D muta…

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pGenLenti murine CD300LF

Plasmid

#235668

Purpose

3rd Generation lentiviral vector for expression of murine CD300LF. Murine CD300LF is the receptor for murine norovirus, and expression confers susceptibility to this virus. Puromycin resistance.

Depositor

Edward Emmott

Article

Frazer J.T. et al bioRxiv 2026.03.19.712921; doi: 10.64898

Insert

Murine CD300LF (Cd300lf Mouse)

Use

Lentiviral

Promoter

CMV

Available Since

March 24, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pPig-hEF1a-Cry2PHR-LRP6c-Puro-Blind

Plasmid

#249712

Purpose

Expresses optogenetic LRP6 in mammalian cells; no fluorescent proteins. Plasmid contains a puromycin selection marker.

Depositor

Max Wilson

Article

Rosen et al bioRxiv [Preprint]. 2025 May 23:2025.02.

Inserts

Cry2-LRP6 (CRY2, LRP6 Mustard Weed, Human)
P2A-PuroR

Expression

Mammalian

Promoter

hEF1a

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A)

Plasmid

#218776

Purpose

Expresses dCAS9-DNMT3A-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A-DNMT3A (dCas9-3A-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A-KRAB (dCas9-3A-KRAB)

Plasmid

#218781

Purpose

Expresses dCAS9-DNMT3A-KRAB fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A-3L)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-mutDNMT3A(R882H)(dCas9-mut3A)

Plasmid

#218788

Purpose

Expresses dCAS9- R882H Mutated DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-MutDNMT3A (dCas9-Mut3A (R882H))
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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