We narrowed to 3,543 results for: biorxiv
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Plasmid#216412PurposeExpresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.DepositorInsertoROS-HT_LF(C199S)
ExpressionMammalianPromoterCMVAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxEXPr6
Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJQR3
Plasmid#218289PurposeIntroduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6DepositorInsertHO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036WExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEdv1
Plasmid#218283PurposeUse together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locusDepositorInsertHph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEu2
Plasmid#218282PurposeUse together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locusDepositorInsertHO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJE13HD7
Plasmid#218281PurposeUse together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.DepositorInsertHO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)
Plasmid#208869PurposeFor mammalian expression of NAP1 (delta-TBK1)DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)
Plasmid#208872PurposeFor expression in E. coli of NAP1 (delta-NDP52)DepositorInsertNAP1 (AZI2 Human)
TagsMBP-TEVExpressionBacterialMutationS37K/A44E (disrupts NDP52 binding)Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)
Plasmid#208873PurposeFor expression in E. coli of NAP1 (delta-TBK1)DepositorInsertNAP1 (AZI2 Human)
TagsMBP-TEVExpressionBacterialMutationL226Q/L233Q (disrupts TBK1 binding)Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor
Plasmid#211905PurposeVector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertDARIC-CD19-scFv
UseAAVExpressionMammalianPromoterhPGK1Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTij_sg_mmu_Med1_N_terminal
Plasmid#197870PurposeCRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCsDepositorAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1d63C + VP2C-GFPdeg
Plasmid#182481PurposeYIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-GFPdeg
deltaVP1d63C
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (M)
Plasmid#182434PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (G)
Plasmid#182476PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-4-IRES-mScarlet
Plasmid#209410PurposeExpression of wild-type neonatal isoform of human SCN2A.DepositorInsertSCN2A Variant 4, stabilized (SCN2A Human)
ExpressionMammalianMutationIVS intron inserted after bp 4551 of the SCN2A OR…PromoterCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
prhaBAD-anti-mouse-Nb-Hia5
Plasmid#239626PurposeRhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferaseDepositorInsertHia5
Tags6HIS, MBP, TEV, and anti-Mouse NanobodyExpressionBacterialMutationCodon optimized for bacterial expressionPromoterrhaBADAvailable SinceJuly 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
VPS13B^GFP
Plasmid#224588Purposecodon optimized GFP tagged VPS13B for expression in mammalian cellsDepositorAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvHA
Plasmid#234991PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinsDepositorInsertNA-3C-myc-scfvHA
ExpressionMammalianAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS
Plasmid#239020PurposeCo-overexpression of Rhobin2-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling markerDepositorInsertRhobin2-H2B (H2BC21 Synthetic, Human)
TagsTagBFP and mNeonGreen3KExpressionMammalianPromoterCMV promoterAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only