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Plasmid#62127PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only
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pMuLE ENTR CMV loxP L5-L2
Plasmid#62094PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site flanked by loxP sites. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseCre/Lox; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
KZ001: pMVP (L3-L2) P2A-eYFP + polyA
Plasmid#121770PurposepMVP L3-L2 entry plasmid, contains eYFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest.DepositorInsertP2A-eYFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 R133A/P139A (#626)
Plasmid#99946Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationR133A/P139APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LA601: pMVP (L3-L2) P2A-Puro + WPRE
Plasmid#121785PurposepMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectorsDepositorInsertP2A-Puro + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ501: pMVP (L3-L2) P2A-Puro + polyA
Plasmid#121780PurposepMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.DepositorInsertP2A-Puro + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 stuffer sgRNA scaffold L5-L4
Plasmid#62129PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
33-G-X1-pEF1-puro
Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_E323K_TEV
Plasmid#47699PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323KDepositorInsertMaltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged glutamic acid 323 to lysinePromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT S6K (E389-deltaCT)
Plasmid#58507PurposeGateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCTDepositorInsertRps6kb1 (Rps6kb1 Rat)
UseEntry vectorTagsHAExpressionMutationT389E, stop codon at amino acid 402PromoterAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-SLC35B2-FLAG
Plasmid#154863PurposeTransient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationctcctggtgcagtacttc => ttattagtccaatattttPromoterCMVAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK-miR-30 L5-L4
Plasmid#62119PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF3
Plasmid#67308PurposehArf3 with stop codon in Gateway Entry vectorDepositorInsertARF3 (ARF3 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 Luc2 (FF-Luciferase) L5-L2
Plasmid#62170PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and firefly luciferase module. Compatible with MultiSite Gateway cloningDepositorInsertLuciferase
UseLuciferase; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
KY901: pMVP (L3-L2) P2A-mCherry + polyA
Plasmid#121766PurposepMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.DepositorInsertP2A-mCherry + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HD201: pMVP (L3-L2) IRES-eGFP + polyA
Plasmid#121747PurposepMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.DepositorInsertIRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
33-G-X5-pEF1/V5-His
Plasmid#111605PurposeExpress human 33-G-X5 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
LA001: pMVP (L3-L2) P2A-mCherry + WPRE
Plasmid#121774PurposepMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-mCherry + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PMYT1_HUMAN_D0
Plasmid#79687PurposeThis plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPMYT1
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L5-L4
Plasmid#62138PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only