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Plasmid#20745DepositorInsertIRE1 (ERN1 Human)
UseLentiviral; Destination vectorTagsExpressionMammalianMutationK599A, dominant negative kinase mutantPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFLAG/HA-DGCR8
Plasmid#10921DepositorInsertDGCR8 (DGCR8 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKIBra-P2A-mCherry-Puro-sfGFP
Plasmid#115686PurposeKnock-in of P2A-mCherry at the mouse Brachyury (T) geneDepositorInsertP2A-mCherry-loxP-PGK-Puro-sfGFP-loxP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-EGFP
Plasmid#221553PurposeExpresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBlueScript-humanAPOBEC1
Plasmid#134606PurposePlasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activityDepositorArticleInserthumanAPOBEC1 (APOBEC1 Human)
UseTagsExpressionMammalianMutationPromoterBeta actinAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-nucl
Plasmid#113915PurposePlasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP GFP-Stx17TM
Plasmid#45910DepositorInsertSyntaxin 17 (STX17 Human)
UseRetroviralTagsGFPExpressionMutationdeleted amino acids 1-228PromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-mGFP-NCOA4
Plasmid#192802PurposeStable expression of mGFP-NCOA4 in mammalian cellsDepositorInsertNCOA4 (NCOA4 Human)
UseTagsmonomeric EGFP (A206K)ExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-S12
Plasmid#84031PurposeTo episomally express codon optimized Cas9 and chimeric guide RNADepositorInsertshSpCas9
eGFP
UseTags3x FLAGExpressionMammalianMutationPromoterCMV and CMV (downstream of F2A self-cleaving pept…Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE
Plasmid#101104PurposeEncodes dCas9(N) fused to VEGFR2DepositorInsertVEGFR2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPP444
Plasmid#158802PurposePlasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-3
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Rheb1 shRNA #1
Plasmid#26625DepositorInsertRheb1 shRNA (RHEB Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFIV-H1-Puro-hPolß
Plasmid#18663DepositorInsertshRNA specific to human DNA polymerase beta (POLB Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationnonePromoterAvailable sinceOct. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-NLS-LSSmKate2
Plasmid#34586DepositorInsertNLS-LSS-mKate2
UseRetroviralTagsExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…PromoterAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-HaloTag7-NCOA4
Plasmid#192806PurposeStable expression of HaloTag7-NCOA4 in mammalian cellsDepositorInsertNCOA4 (NCOA4 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-AKT1-V5-miniTurbo_IDG-K
Plasmid#189889PurposeGateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-AKT1_IDG-K
Plasmid#189890PurposeGateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-MSCV-4xS1m-GFP-T2A-PU
Plasmid#138581PurposeFor in vivo taggingDepositorTypeEmpty backboneUseLentiviralTagsS1m tagExpressionMutationPromoterMSCVAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gso (Ser)
Plasmid#109353PurposeGsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGso chimera
UseTagsNoneExpressionMammalianMutationChanged Cysteine at position 391 to Serine (Pertu…PromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only