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pYG215
Plasmid#200837PurposeTranscribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.DepositorInsertglmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterP-LlacO-1Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-CDK5RAP3
Plasmid#204566PurposeExpresses GST-CDK5RAP3 in E.coliDepositorInsertCDK5RAP3 (AMY2A Human)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI
Plasmid#202596Purposeplasmid for expressing the DNMT3bCI (catalytic inactive)DepositorInsertH2B-mCherry-T2A-rTetR-DNMT3bCI (Dnmt3b Mouse, Synthetic)
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationP656V, C657DPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_578-675-GB1_AD
Plasmid#148504PurposeBacterial Expression of DmeIF4G_578-675DepositorInsertDmeIF4G_578-675 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_601-634-GB1_AD
Plasmid#148505PurposeBacterial Expression of DmeIF4G_601-634DepositorInsertDmeIF4G_601-634 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_601-675-GB1_AD
Plasmid#148507PurposeBacterial Expression of DmeIF4G_601-675DepositorInsertDmeIF4G_601-675 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_617-634-GB1_AD
Plasmid#148508PurposeBacterial Expression of DmeIF4G_617-634DepositorInsertDmeIF4G_617-634 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_617-675-GB1_AD
Plasmid#148509PurposeBacterial Expression of DmeIF4G_617-675DepositorInsertDmeIF4G_617-675 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-GB1_Z
Plasmid#148178PurposeBacterial Expression of DmThor_50-63DepositorInsertDmThor_50-63 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83_Z
Plasmid#148179PurposeBacterial Expression of DmThor_50-83DepositorInsertDmThor_50-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_63-83-GB1_Z
Plasmid#148181PurposeBacterial Expression of DmThor_63-83DepositorInsertDmThor_63-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-del28-43-V5His6_Z
Plasmid#148157PurposeInsect Expression of Dm4E-T_1-511-del28-43DepositorInsertDm4E-T_1-511-del28-43 (4E-T Fly)
UseTagsExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z
Plasmid#148158PurposeInsect Expression of Dm4E-T_1-511-FWKtoAAADepositorInsertDm4E-T_1-511-FWKtoAAA (4E-T Fly)
UseTagsExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-L36A L69A-V5His6_Z
Plasmid#148159PurposeInsect Expression of Dm4E-T_1-511-L36AL69ADepositorInsertDm4E-T_1-511-L36AL69A (4E-T Fly)
UseTagsExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-Y10AL15A-V5His6_Z
Plasmid#148161PurposeInsect Expression of Dm4E-T_1-511-Y10AL15ADepositorInsertDm4E-T_1-511-Y10AL15A (4E-T Fly)
UseTagsExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z
Plasmid#148162PurposeInsect Expression of Dm4E-T_1-511-YLLLtoAAAADepositorInsertDm4E-T_1-511-YLLLtoAAAA (4E-T Fly)
UseTagsExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_1-417-CG32016-E_T
Plasmid#147583PurposeInsect Expression of DmCup_1-417-CG32016-EDepositorInsertDmCup_1-417-CG32016-E (cup Fly)
UseTagsExpressionInsectMutation5 silent and three no silent mutations H117R, N14…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only
