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Plasmid#197134PurposeCloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSI-218
Plasmid#197139PurposeAll-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unitDepositorTypeEmpty backboneUseCRISPRTagsSpCas9-PmCDA1-UGIExpressionMammalianMutationSpCas9(D10A)PromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-060
Plasmid#197133PurposeCRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expressionDepositorTypeEmpty backboneUseLentiviralTagsEGFP and mCherryExpressionMammalianMutationMCP(N55K)PromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-193
Plasmid#197137PurposeCloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcodeDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationmCherry(M1V/M9A)PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-119
Plasmid#197135PurposeCloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES
Plasmid#182433PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-FPPS
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (M)
Plasmid#182435PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (G)
Plasmid#182477PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase
Plasmid#191481PurposeLuciferase reporter for SARS-CoV-2 5' UTR ΔSL1DepositorInsertSARS-CoV-2 5' UTR ΔSL1
UseLentiviral and LuciferaseTagsExpressionMutationMissing the stem-loop 1 region of SARS-CoV-2 5…PromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)
Plasmid#190755PurposeExpression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.DepositorUseTagsmCherry2 and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAT +D122H
Plasmid#187426PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorUseTagsExpressionInsectMutationchanged aspartic acid at 129 for histidinePromoterPH and p10Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAT +R111E
Plasmid#187425PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorUseTagsExpressionInsectMutationchanged arginine at 118 to glutamic acidPromoterPH and p10Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
tetO-Fzd1-IRES-tdTomato (TET006)
Plasmid#160510PurposeThis plasmid expresses Fzd1 under the activation of tTA trans-activator along with fluorescent marker tdTomato.DepositorArticleInsertFzd1 (Fzd1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)
Plasmid#133791PurposeU6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018DepositorInsertSt1Cas9 sgRNA entry vector
UseTagsExpressionMammalianMutationV1 sgRNA architecture from Carter et al. biorxiv …PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF159_BRL
Plasmid#225962PurposeCMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.DepositorInsertCMV-Intron-BRL (env protein)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD
Plasmid#225697PurposeLentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE
Plasmid#188757PurposeLentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertZNF10 KRAB (ZNF10 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF160_VSV-G
Plasmid#225963PurposeCMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.DepositorInsertCMV-Intron-VSVG (env protein)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD
Plasmid#214084PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-hP53DDDepositorInsertPEmax-P2A-hP53DD (TP53 Human, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone
Plasmid#216154PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5']DepositorInsertmScarlet with cryptic exon and C-terminal FLAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only