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Plasmid#97423PurposeTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only
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ZsGreen1-cMYC/pLVX-Puromycin
Plasmid#180278PurposePlasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).DepositorInsertZsGreen1-cMYC fusion protein MYC (MYC Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl2-Cb5
Plasmid#18000DepositorInsertER targeted Bcl-2 (BCL2 Human)
UseTagsGFPExpressionMammalianMutationBcl-2's C terminal targeting sequence replac…PromoterCMVAvailable sinceJune 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPtPBR11
Plasmid#80386PurposeEpisome vector for Phaeodactylum tricornutum (pBR322-based)DepositorTypeEmpty backboneUseSynthetic Biology; : episomal vector for phaeodac…TagsExpressionMutationPromoterAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl2-Maob
Plasmid#18001DepositorInsertMitochondria-targeted Bcl-2 (BCL2 Human)
UseTagsGFPExpressionMammalianMutationBcl-2's C terminal replaced with mitochondri…PromoterCMVAvailable sinceJune 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorInsertSARS-CoV-2 S (S SARS CoV 2)
UseTags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids del…PromoterCMVAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
W118-1_hTGM2_C277S-Flag
Plasmid#180412Purposelentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tagDepositorInsertTGM2_C277S (TGM2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationC277SPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LBR pEGFP-N2 (646)
Plasmid#61996Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertLBR (LBR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Perox-GA
Plasmid#209868PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3DepositorInsertddGFP A
UseTagsTargeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc1/bC
Plasmid#74049Purposeretroviral expression plasmid for human NFATc1/bCDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsno tagExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
h-gamma-ENaC Myc
Plasmid#83428Purposefull length human gamma ENaC with myc tagDepositorInserthuman gamma ENaC (SCNN1G Human)
UseTagsMycExpressionMammalianMutationP502APromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBIS
Plasmid#35150DepositorInsertAtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLacUV5,Available sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF-HA-NBP1 (P#1261)
Plasmid#14586DepositorInsertATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-mCherry-24xMBS
Plasmid#86242PurposeMiddle entry vector for generating MBS tagged RNAs with a fluorescent protein reporterDepositorTypeEmpty backboneUseUnspecifiedTagsmCherry-24xMBSExpressionMutationPromoterAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Halo-TOMM20-N-10
Plasmid#123284PurposeLocalization: Outer Mitochondrial MembraneDepositorInsertTOMM20 (TOMM20 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-V5/HIS
Plasmid#201987Purposeexpression of human PDGFRA receptor tyrosine kinase in mammalian cellsDepositorInsertPDGFRA receptor tyrosine kinase (PDGFRA Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
L304-EGFP-Tubulin-WT
Plasmid#64060PurposeEGFP fused at the N-terminus of tubulin; lentiviral constructDepositorInserttubulin
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCer-C3-Plk1
Plasmid#68132PurposeExpression of Cerulean-tagged Plk1 in mammalian cellsDepositorInsertPolo-like kinase 1 (PLK1 Human)
UseTagsCeruleanExpressionMammalianMutationwild-typePromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-pBAD
Plasmid#54542PurposeLocalization: Protein Expression Vector , Excitation: 383, Emission: 448DepositorTypeEmpty backboneUseTags6xHis and EBFP2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL552
Plasmid#68407PurposeDonor empty vector with floxed puromycin resistance gene expression cassetteDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLTR.gp140/EGFP.Revdelta38/DsRed
Plasmid#115775PurposeHIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.DepositorInsertEnvelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
UseTagsEGFP; dsReDExpressionMammalianMutationgp140 uncleaved (1190-91aa, KR > TG; truncated…PromoterHIV-1 (LTR)Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGW-GOI-TurboID
Plasmid#209378PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA
Plasmid#108747PurposeExpresses N-terminus to aa27 of Flp recombinase fused to GID1 CIDDepositorInsertFlp recombinase fragment N-terminus to aa27
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX401-INK4A
Plasmid#121919PurposeDoxycycline-inducible expression of p16-INK4A product of CDKN2ADepositorInsertp16-INK4A (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE Tet ONAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCer-TG2-YFP
Plasmid#85471PurposeTG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRETDepositorInserthTG2 (TGM2 Human)
UseTagsEYFP and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-alpha
Plasmid#88846PurposeCRISPR KO of Trp53DepositorInsertTrp53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-LifeAct-GFP
Plasmid#190199PurposeExpresses the small actin binding peptide, LifeAct, with a GFP reporterDepositorInsertLifeAct-eGFP
UseAAVTagsGFPExpressionBacterial and MammalianMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR8-YFP
Plasmid#13024DepositorInsertToll-Like Receptor 8 (TLR8 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only