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Plasmid#143655PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI3 (GLI3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-ROC1
Plasmid#19897DepositorInsertRING finger protein 1 (ROC1) (RBX1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AsCas12f1
Plasmid#171614PurposeAsCas12f1-based Human cell genome editingDepositorInsertAsCas12f1 and sgRNA_v1
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXK2
Plasmid#153133PurposeExpression of Flag tagged human FOXK2 in mammalian cellsDepositorInserthuman FOXK2 (FOXK2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRV1
Plasmid#180515PurposeAgrobacterium tumefaciens-based expression of TRV1DepositorInsertTobacco rattle virus RNA1
UseTagsExpressionPlantMutationPromoter35S CaMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Elongin B-pcDNA3.1(+)-Zeo
Plasmid#20000DepositorInserttranscription elongation factor B (ELOB Human)
UseTagsHA-tagExpressionMammalianMutationwild typePromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NOD2-EGFP
Plasmid#131207Purposemammalian expression, tet RDepositorInsertNOD2 (NOD2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FTO-dCas9
Plasmid#134781PurposeExpression fusion protein FTO-dCas9 in Mammalian cellsDepositorInsertFTO and inactive dCas9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-SpRY
Plasmid#161520PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoterDepositorInsertzSpRYCas9
UseCRISPR; Gateway compatible zsprycas9 entry cloneTags3X FLAG, NLS and NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-pBAD
Plasmid#54519PurposeLocalization: Protein Expression Vector , Excitation: 485, Emission: 507DepositorTypeEmpty backboneUseTags6xHis and sfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX-TRV2
Plasmid#180516PurposeAgrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinkerDepositorInsertTobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
UseTagsExpressionPlantMutationPromoter35S CaMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmGFP11C-Tau
Plasmid#71434PurposeSplit GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433DepositorInsertTau (0N4R) (MAPT Human)
UseTagsGFP (213-228)ExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 D210N
Plasmid#174448PurposeExpress GFP-tagged RNase H1 D210N in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
UseTagsHis-tag, GFPExpressionBacterialMutationD210NPromoterT7 promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianMutationPromotertet-onAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA
Plasmid#163476PurposeDirect-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpAInsertiCre
UseAAVTagsExpressionMutationPromoterBeta Globin minimal promoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_blast_MCS_Nard_GFP_LAMIN
Plasmid#167340PurposeExpresses GFP fused to lamin A (LA) in mammalian cellsDepositorInsertLamin A (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_NEUROD1_P2A_Hygro_Barcode
Plasmid#120466PurposeBarcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertNEUROD1 (NEUROD1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only