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Plasmid#205462Purposelentiviral plasmid for expression of HLA-G SCTDepositorInsertHLA-G SCT (HLA-G Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-TRE-AFOS
Plasmid#197079PurposeA doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.DepositorInsertAFOS (Fos Mouse)
UseLentiviralTagsFlag and T7ExpressionMutationPromoterTREAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His
Plasmid#83453PurposeFull-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)DepositorInsertpolycystin-1 (Pkd1 Mouse)
UseTags2xMyc, 3xFlag, and 6xHisExpressionMammalianMutationS4144DPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MK14_HUMAN_D0
Plasmid#79680PurposeThis plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK14
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PGAM1_sgRNA1
Plasmid#201614PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPGAM1 (PGAM1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn-HpaBC
Plasmid#100949PurposeExpression of RgTALsyn, HpaB, and HpaC from inducible T7 promoterDepositorInsertsRgTALsyn
HpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf37
Plasmid#12725PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf37
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf48
Plasmid#12736PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf48
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-KPTN
Plasmid#87042Purposemammalian expression of KPTNDepositorInsertKPTN (KPTN Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin3
Plasmid#72592Purposeoverexpression, codon optimized for mammalian expressionDepositorInsertSESN3 (SESN3 Human)
UseTagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
dxCas(3.6)-VPR
Plasmid#108388PurposeMammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminusDepositorInsertdxCas(3.6)-VPR
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE3∆UGI
Plasmid#96943PurposeThe plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).DepositorInsertBE3∆UGI
UseTagsHis6 tagExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
STK3_HUMAN_D0
Plasmid#79689PurposeThis plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK3 (STK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG
Plasmid#74261PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.Y591X truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ABE-NT-sgRNA
Plasmid#112734PurposeAAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNADepositorInsertABE-NT-sgRNA
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PGAM1_sgRNA2
Plasmid#201615PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPGAM1 (PGAM1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (NZF1, aa 1-33)
Plasmid#65437PurposeExpresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coliDepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GSTExpressionBacterialMutationonly codes for amino acids 1-33 of TRABID, which …PromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-ITFG2
Plasmid#87045Purposemammalian expression of ITFG2DepositorInsertITFG2 (ITFG2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-Ataxin3Q22
Plasmid#22117DepositorInsertAtaxin 3 (ATXN3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only