Showing: 2981 - 3000 of 4263 results
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Plasmid#169602PurposeTier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).DepositorInsertcAMP response element-controlled NanoLuc Luciferase expression cassette
UseTagsExpressionMammalianMutationPromoterPCREm4 / PPGKAvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-FE-PuroR
Plasmid#186746PurposeModified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpinDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2a
Plasmid#186818PurposeFluorescent reporter for Camk2a expressionDepositorInsertCamk2a promoter (Camk2a Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2a promoterAvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc
Plasmid#177716PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targetingDepositorInserthFluc
UseTagsMitochondrial Targeting Sequence MTS SSExpressionBacterial, Mammalian, and Y…MutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV: Flag-Akaluc_SV40:PuroR
Plasmid#183048PurposeExpresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.DepositorInsertAkaLuc
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
shRNA-Ratio-NegCon
Plasmid#183554PurposeDesigned to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)DepositorInsertLuciferase shRNA
UseTagsExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
IRE-SunTag
Plasmid#196920PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
IRE-nonOpt-TREAT
Plasmid#196922PurposeThe codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.DepositorInsertnon-optimal Renilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
IRE-TPI-wildtype-TREAT
Plasmid#196923PurposeThe coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORFDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Let7-IRE-TREAT
Plasmid#196925PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Let7-SunTag
Plasmid#196928PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Let7R-SunTag
Plasmid#196929PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
miR21-IRE-TREAT
Plasmid#196930Purposemir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
miR21mutant-IRE-TREAT
Plasmid#196931PurposeMutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Let7R-IRE-TREAT
Plasmid#196934PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDMS151A_Tet-On-3G
Plasmid#191577PurposeClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).DepositorInsertTet-On(R) 3G
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLX304_CMV::mCherry-LgBiT-FLAG-NES
Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi1-91-NLuc
Plasmid#207148PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI1
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi2-91-NLuc
Plasmid#207149PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI2
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)QPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI3
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 2981 - 3000 of 4263 results