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BMPR1A gRNA (BRDN0001487137)

Plasmid

#77958

Purpose

3rd generation lentiviral gRNA plasmid targeting human BMPR1A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BMPR1A (BMPR1A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ITPKB gRNA (BRDN0001147872)

Plasmid

#76871

Purpose

3rd generation lentiviral gRNA plasmid targeting human ITPKB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ITPKB (ITPKB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ITPKB gRNA (BRDN0001144973)

Plasmid

#76872

Purpose

3rd generation lentiviral gRNA plasmid targeting human ITPKB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ITPKB (ITPKB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ITPKB gRNA (BRDN0001146473)

Plasmid

#76873

Purpose

3rd generation lentiviral gRNA plasmid targeting human ITPKB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ITPKB (ITPKB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ITPKB gRNA (BRDN0001145261)

Plasmid

#76874

Purpose

3rd generation lentiviral gRNA plasmid targeting human ITPKB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ITPKB (ITPKB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387E

Plasmid

#59906

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387E, phospho-dominant

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-LA

Plasmid

#206197

Purpose

Expresses GFP-tagged human Lamin-A protein by the constitutive CMV promoter in mice

Depositor

Yuxuan Guo

Article

Sun et al J Am Coll Cardiol Basic Trans Science. n

Insert

Lmna (LMNA )

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-GFP-LA

Plasmid

#206198

Purpose

Expresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoter

Depositor

Yuxuan Guo

Article

Sun et al J Am Coll Cardiol Basic Trans Science. n

Insert

Lmna (LMNA Human)

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

Tnnt2

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-ChloC

Plasmid

#179841

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC)

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-iChloC

Plasmid

#179842

Purpose

donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

improved chloride-conducting ChR (iChloC)

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-luciferase

Plasmid

#179844

Purpose

donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A mLIR

Plasmid

#215506

Purpose

Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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mAzGreen-Cyclin D2-NeoR

Plasmid

#215062

Purpose

Expresses mAzGreen-Cyclin D2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Tags

mAzGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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SS-Cyclin D2

Plasmid

#215115

Purpose

Expresses SS-Cyclin D2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Tags

SS (STREP-STREP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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mAzGreen-Cyclin D2-PuroR

Plasmid

#215058

Purpose

Expresses mAzGreen-Cyclin D2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

mAzGreen

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-Cyclin D2

Plasmid

#215051

Purpose

Expresses EGFP-Cyclin D2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

Plasmid

#204696

Purpose

This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of the amino acids 355-505 of NUT

Promoter

CMV

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1101

Plasmid

#143925

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GTF2IRD1 (GTF2IRD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1100

Plasmid

#143924

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GTF2IRD1 (GTF2IRD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF3339

Plasmid

#144815

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HDAC3 (HDAC3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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