-
Plasmid#67571PurposeExpresses GFP and FLAG-tagged AKAP-Lbc in mammalian cellsDepositorInsertA-kinase anchoring protein Lbc (AKAP13 Human)
UseTagsFLAG and GFPExpressionMammalianMutationR2604G in AKAPPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
ER_K20
Plasmid#133861PurposeExpression of ER-targeted Ribosome stalling reporterDepositorInsertER_K20
UseTagsEGFP and RFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-zeo largeTcDNA
Plasmid#1779DepositorInsertlarge T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9.452sub.LUNG1
Plasmid#184592Purposenon-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injectionDepositorInsertSynthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A
Plasmid#177335Purposebacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinaseDepositorInsertSARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
UseTagsFactor Xa site-3xFlag-Myc-6xHisExpressionBacterialMutationsilent mutation C to T at position 10546 (elimina…PromoterT7Available sinceNov. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXPR_053
Plasmid#113591PurposeConstitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cellsDepositorInsertsgRNA cloning site
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AHSG-bio-His
Plasmid#52175PurposeExpresses full-length Alpha-2-HS-glycoprotein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertAHSG (AHSG Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail WT
Plasmid#16225DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mScarlet-MAP4-MTBD
Plasmid#171488PurposeT7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNADepositorInsertMAP4-MTBD (Map4 Mouse)
UsePgehmeTagsmScarletExpressionMutationAminoacids 658-1126PromoterT7Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse p19ARF-IRES-GFP
Plasmid#107147PurposeMammalian expression of mouse ArfDepositorInsertArf [alternative reading frame] (Cdkn2a Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)
Plasmid#153005PurposeExpresses high-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (C) (G79)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Cytochrome C
Plasmid#41181DepositorInsertCytochrome C (CYCS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-STX17ΔNTD
Plasmid#89941PurposeExpresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cellsDepositorInserthuman syntaxin17 lacking N-terminal domain (STX17 Human)
UseRetroviralTagsGFPExpressionMutationdeleted amino acids 1-161PromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-mTagBFP2
Plasmid#96935PurposeRetroviral plasmid containing mTagBFP2 for labeling of cellsDepositorInsertMonomeric Tag Blue Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcdna6.2- myc ULK1 k46I
Plasmid#27630DepositorInsertmouse myc ULK1 K46I (Myc Mouse)
UseTagsmycExpressionMammalianMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta1(617-949)-GFP
Plasmid#100732PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP1B1 (AP1B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BTRC_WT
Plasmid#82244PurposeGateway Donor vector containing BTRC , part of the Target Accelerator Plasmid Collection.DepositorInsertBRTC (BTRC Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-tTAc
Plasmid#172123PurposeConstitutive expression of InteinC-tTACDepositorInsertInteinC-tTAc
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUE2m
Plasmid#129350PurposeATP measurementDepositorInsertQUEEN-2m
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.tgoGAP1
Plasmid#78737PurposeExpression of Ca2+ sensor GAP1 in the trans Golgi apparatusDepositorInserttgoGAP1
UseTagsN terminal 81 amino acids of human galactosyltran…ExpressionMammalianMutationD117A/D119A/D163A in aequorin moietyPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti.pFOXA2.GFP
Plasmid#59404Purposeexpresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during developmentDepositorInsert400 bp FOXA2 promoter, eGFP (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpFOXA2Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mClover3
Plasmid#74236PurposeConstitutive expression of mClover3 in bacteriaDepositorHas ServiceCloning Grade DNAInsertmClover3
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
deltaNp63alpha-FLAG
Plasmid#26979DepositorInsertΔNP63alpha (TP63 Human)
UseTagsFLAGExpressionMammalianMutationdominant negative isoform of p63; aa 1–587PromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP::L4440
Plasmid#11335DepositorInsertGFP
UseRNAiTagsExpressionWormMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
VPS13D^EGFP
Plasmid#174109PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.DepositorInsertCodon optimized VPS13D
UseTagsEGFPExpressionMammalianMutationWith an internal EGFP tag in aminoacid position 1…PromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB1691-Nluc
Plasmid#204324PurposeGPCR/G protein BRET biosensorDepositorInsertKB1691-Nluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TFAP4
Plasmid#110156PurposeExpression of human TFAP4 in mammalian cellsDepositorInsertTFAP4 (transcription factor AP-4) (TFAP4 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL4B
Plasmid#19922DepositorInsertCullin 4B (CUL4B Human)
UseTags3xmycExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12C
Plasmid#58901PurposeExpresses human Kras with a G12C mutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12C mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)
Plasmid#191389PurposeDonor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locusDepositorInserthCdt1(1-100)
UseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE4
Plasmid#195369PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInserthA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP25-1A(sfGFP)
Plasmid#126972PurposeLevel 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-p53 WT
Plasmid#49242Purposeexpresses human wt p53 and EGFP in mammalian cellsDepositorInsertp53 (TP53 Human)
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3 RARE-d2EGFP
Plasmid#183055PurposeDestabilized EGFP fluorescent reporter for Retinoic Acid activityDepositorInsertd2EGFP
UseTagsExpressionMutationPromoterRARE, SV40Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_neomycin
Plasmid#65307PurposeLuminal ER hook only (RUSH system)DepositorInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
UseLentiviralTagsKDEL motif and Signal SequenceExpressionMutationPromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-KPTN
Plasmid#87041Purposemammalian expression of KPTNDepositorInsertKPTN (KPTN Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70
Plasmid#62915PurposeCreating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-p65AD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL4393 (pClpX_Eco)
Plasmid#200907PurposeExpresses human codon-optimized E. coli ClpX from a CMV promoter.DepositorInsertEcoClpX
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-p21
Plasmid#171122PurposeDoxycycline inducible expression of p21DepositorInsertp21 (CDKN1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-AdEvolve-DEST
Plasmid#131692PurposeA version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_Ado1.0
Plasmid#153288PurposeExpress the adenosine sensor GRAB_Ado1.0 in astrocytesDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Y1214F-VEGFR2-mCherry
Plasmid#139609PurposeExpresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cellsDepositorInsertKDR (KDR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
5xNF-kb_RE::RedF
Plasmid#124530PurposeNFKB RedFirefly luciferase reporterDepositorInsertNF-κβ RedF reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2885
Plasmid#144256PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI2 (GLI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_TIA1
Plasmid#106095Purposebacterial protein expression of TIA1DepositorInsertTIA1 (TIA1 Human)
UseTags6xHIs-thrombin and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAX FLAG APP
Plasmid#30154DepositorInsertAmyloid Precursor Protein (APP Human)
UseTagsFLAGExpressionMammalianMutationwild type splice variant 751PromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
rAAV9-CAP-in-cis-Lox-AAV
Plasmid#205990PurposerAAV9 cap acceptor vector for CREATE librariesDepositorInsertrAAV9 cap acceptor vector for CREATE libraries
UseAAVTagsExpressionMutationDeletion between XbaI and AgeI sitesPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-mCh-Cry2olig
Plasmid#101224PurposeFUS(1-214) fused to mCh-Cry2oligDepositorInsertFUS(aa1-214) (FUS Human)
UseLentiviralTagsmCherry-Cry2oligExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only