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sfGFP-SasG(4G)-mCherry

Plasmid

#206946

Purpose

Mammalian expression of heterobifunctional SasG linkers (4G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (4G)-mCherry

Use

Tags

mCherry and sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(2G)-mCherry

Plasmid

#206944

Purpose

Mammalian expression of heterobifunctional SasG linkers (2G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (2G)-mCherry

Use

Tags

mCherry and sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-AQNAT

Plasmid

#198211

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_AQNAT

Use

Tags

6x His Tag and AQNAT mutated (non-permissible) gl…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-MOOR

Plasmid

#198212

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_MOOR

Use

Tags

6x His Tag and MOOR glycosylation tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-MOORmut

Plasmid

#198213

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_MOORmut

Use

Tags

6x His Tag and mutated (non-permissible) MOORmut …

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-sfGFP

Plasmid

#179782

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

sfGFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-19 TC-sfGFP-tag3

Plasmid

#194633

Purpose

fluorescent protein with cationic peptide tag and tetracysteine tag

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-sfGFP-K(GGSKKRKKR)3

Use

Tags

KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-2 sfGFP-tag2

Plasmid

#194617

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

sfGFP-K(GGSKKRKKR)2

Use

Tags

KGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-1 sfGFP-tag1

Plasmid

#194616

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

sfGFP-KGGSKKRKKR

Use

Tags

KGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut2

Plasmid

#180425

Purpose

mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3 Gmut-msfGFP

Plasmid

#180326

Purpose

mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i3 W502A, H512D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut1

Plasmid

#180316

Purpose

mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3 NCmut-msfGFP

Plasmid

#180325

Purpose

mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i3 I263D, M270D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pxyl-ftsZ-sfGFP

Plasmid

#174505

Purpose

Integrated plasmid harboring ftsZ-sfGFP gene, inducible by xylose (sequence of sfGFP optimized)

Depositor

Suliana Manley

Article

Mahecic et al Nat Methods. 2022 Sep 8. pii: 10.1038/s4

Insert

ftsZ

Use

Tags

superfold GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-sfGFP

Plasmid

#180756

Purpose

pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Use

Tags

strep tag

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S111F-msfGFP

Plasmid

#180340

Purpose

mammalian expression of human SEPT9_i1 S111F fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S111F

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R106W-msfGFP

Plasmid

#180339

Purpose

mammalian expression of human SEPT9_i1 R106W fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R106W

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 NCmut1-msfGFP

Plasmid

#180329

Purpose

mammalian expression of human SEPT9_i1 NCmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R289A R290A K291A K294A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 D10-25-msfGFP

Plasmid

#180334

Purpose

mammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 D10-25

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 DN7-msfGFP

Plasmid

#180335

Purpose

mammalian expression of human SEPT9_i1 DN7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 DN7

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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